Molecular Plant Breeding 2016, Vol.7, No.20, 1
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1.1 Cluster analysis
Clustering indicates that all breeding lines were grouped into nine clusters. Solitary line was found in the first,
second, third, and fifth cluster, where as fourth, sixth, seventh, eighth, and ninth cluster consisted of 4, 3, 13, 20,
and 20 breeding lines, respectively (Table 3 and Figure 1).
Table 3 Grouping of oilseed Brassica breeding lines in different clusters
Cluster
No of breeding lines
Breeding lines code
I
1
48
II
1
25
III
1
64
IV
4
57,24,26,8
V
1
51
VI
3
43,47,39
VII
13
21,31,21,34,32,52,42,16,56,27,46,37,14
VIII
20
50,10,40,6,62,45,63,30,13,9,17,12,4,15,11,33,7,60,61,3
IX
20
49,54,59,35,53,29,28,20,23,55,36,38,18,58,44,41,5,2,19,1
1.2 Principal component analysis
Principal component analysis indicates that 71.1% of the variation was explained by the first three principal
components (Table 4). Characters such as days to flowering, days to maturity, number of secondary branches/plant,
number of pods/plant, and yield/plot showed the major positive contribution under the first principal component in
which about 32% of the variation explained, where as 1 000 seed weight showed negative weight under this
principal component. Plant height, number of primary branches/plant, and 1 000 seed weight had the major
positive contribution in the second principal component, whereas number of secondary branches/plant and number
of pods/plant recorded negative weight under this component by which 26.4% of the variation was explained. In
principal component III (12.8% of variation), plant height, number of primary branches/plant, number of
secondary branches/plant, number of pods/plant, and 1 000 seed weight had positive weight while days to
flowering, days to maturity, and yield/plot showed negative weight.
Table 4 Component scores of the first three principal components of the breeding lines
Characters
1
2
3
1
0.4339
0.2693
-0.3686
2
0.4371
0.1632
-0.4839
3
0.1637
0.5204
0.2162
4
0.2707
0.3995
0.4557
5
0.4065
-0.3812
0.3071
6
0.4364
-0.2762
0.4434
7
0.3157
0.0644
-0.1203
8
-0.2554
0.4946
0.2648
Eigen value
2.556
2.113
1.020
Variance (%)
31.951
26.406
12.751
Cumulative (%)
31.951
58.357
71.108
1.3 Genetic parameters estimate
High phenotypic and genotypic variances was recorded for yield/plot, number of pods/plant, and plant height
(Table 5). Maximum phenotypic and genotypic coefficients of variation were recorded by number of secondary
branches/plant (0.680 and 0.625, respectively) followed by yield per plot (0.425 and 0.389, respectively) and 1
000 seed weight (0.232 and 0.225, respectively). Heritability in the broad sense estimates were grouped into
