Molecular Plant Breeding 2016, Vol.7, No.17, 1-7
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substrate, peroxidase activities were enhanced in chilling stressed-plant as compared with control plant (Lee and
Lee, 2000). Comparing the peroxidase profiles between control plants and those treated under cold stress using
native-page, the pattern of isoenzyme showed that cold treatment induced a high peroxidase activity. This activity
increase slightly in roots tissues (Figure 2, A) than in aerial (leave and shoot) tissues (Figure 2, B) in both
ecotypes. The peroxidase activities were more pronounced when cold stress is maintained in the time. This trend
was observed in tolerant accession Cil 126 when plantlets were subjected at 6 days of stress. In contrast, in
sensible accession Cil 123 peroxidase activities were slightly maintained (Figure 3: A and B). The same results
were observed in chickpea (
Cicer orientum
L.) (Nazari et al., 2012). It was found that the number of bands
(named POD–Rfs) was higher (five) in tolerant (Cil 126) (Figure 2) than in sensible (Cil 123) (Figure 3) with only
four bands (POD 1 to POD 4). Moreover, results revealed that under cold treatment the intensity of bands were
more pronounced in roots (Figure 2, A and Figure 3, A) than in aerial tissues (Figure 2, B and Figure 3, B). As
showed in Figure 2A the intensity of POD 5-Rf_0.89 of Cil 126 and in Figure 3A POD 4-Rf_0.69 of Cil 123 in
root was increased in cold treated plants in comparison of aerial tissues in both tolerant and sensible accession.
Mohammadian et al., (2012) showed that in tissues of three cultivars of lemon (
Citrusunshiu
,
Citrussinensis
and
Citrus lemon
) the intensity of peroxidise bands is low in the sensible cultivars and is more important with 3intense
bands in both tolerant cultivars (lemon
Citrussinensis
and
Citrus lemon
) under a cold treatment. Furthermore, Lee
and Lee (2000) have observed this tendency. These authors, analyzing the changes of antioxidant enzyme isoforms
against chilling stress in the leaves of cucumber (
Cucumis sativus
L.), found five APX isoforms were presented in
the leaves of cucumber. Furthermore, these authors showed that intensities of APX-4 and -5 were enhanced by
chilling stress, whereas that of APX-3 was significantly increased in the post stress periods after chilling stress.
On the other hand, Azzeme et al., (2016) noted that POD activity increased compared to control under different
severity of drought in both tissues (leaves and roots) which showed that POD is an essential enzyme in scavening
H
2
O
2
in the oil palm seedlings. Mirzaee et al., (2013) reported an initial increase and later decrease in antioxidant
activities in leaves and roots tissues of
Brassica napus
L. compared to control plants with the increasing duration
of stress indicating the unbalance of the active oxygen metabolism system. The high accumulation of active
oxygen was able to initiate and accelerate lipid peroxidation. Nevertheless, when the levels of the active oxygen
exceeded the ability of antioxidant system to cope with them, damage to cellular components occurred (Cao et al.,
2011).
Figure 2 Peroxidase isoenzyme patterns in
M. ciliaris
Krockers
.
(Cil 126) seedlings untreated (controls): (T02, T04,
and T06) and treated with different durations (T2, T4, and T6)
under cold stress
Notes: A: Root vegetative tissues and B: Aerial vegetative
tissues.
Figure 3 Peroxidase isoenzyme patterns in M. ciliaris
Krockers. (Cil 123) seedlings untreated (controls): (T02, T04,
and T06) and treated with different durations (T2, T4, and T6)
under cold stress
Notes: A: Root vegetative tissues and B: Aerialvegetative
tissues.
Notes: A: Root vegetative tissues and B: Aerial vegetative
tissues.
