Molecular Plant Breeding 2016, Vol.7, No.17, 1-7
1
Research Report Open Access
Aerials and Roots Vegetative Tissues Differ in Guaiacol Peroxidases Responses
to Cold Stress in Two Contrasting
Medicago ciliaris
Populations
Nourredine Yahia , Asma Bouira, Chahrazed Cheriet, Fatima Zohra Fyad-Lamèche
Département de Biologie, Facultédes Sciences de la Nature et de la Vie, UniversitéOran1 (Ahmed Ben Bella), BP. 16 Es-senia 31000 Oran 1, Algeria
Corresponding author Email
Molecular Plant Breeding, 2016, Vol.7, No.17 doi
Received: 17 Feb., 2016
Accepted: 02 Apr., 2016
Published: 19 Apr., 2016
Copyright © 2016
Nourredine et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Nourredine Y., Asma B., Chahrazed C., and Fatima Z.F.L., 2016, Aerials and Roots Vegetative Tissues Differ in Guaiacol Peroxidases Responses to Cold Stress
in Two Contrasting
Medicago ciliaris
Populations,Molecular Plant Breeding, 7(17): 1-7 (doi
Abstract
Effect of cold on antioxidant responses in aerial (shoots and leaves) and roots tissues of two contrasting accessions of
M.
ciliaris
Krockers (Cil 126, tolerant and Cil 123, sensible) were investigated. Ten-day-old grown seedlings were subjected at different
periods of cold acclimation (4
℃
) 2, 4, and 6 days. Peroxidases (POD) (EC 1.11.1.7) activities and isoenzymes expression of plantlets
treated and control (23
℃
) of the aerial (shoot+leaves) and root vegetative tissues were carried. Global peroxidase activity under low
temperature stress was higher in tolerant ecotype than sensible one. The same trend was also observed at expressed isoenzymes. On the
other hand, whether in tolerant or sensitive, activity and isoenzymes peroxidase was more pronounced at the roots than in aerials tissues
system.
Keywords
Peroxidase activity; Isoenzymes; Cold stress;
M. ciliaris
; PAGE electrophoresis
Introduction
Cold stress is one of the major factors limiting agricultural productivity, consequently the plants evolved a numerous
adaptive responses to cope with environmental stresses. Agricultural yield losses due to abiotic environmental
stresses are obvious and have been well-documented (Cramer et al., 2011). Abiotic and biotic stress often causes a
series of morphological, physiological, biochemical and molecular changes that unfavourably affect plant growth,
development and productivity. Drought, salinity, extreme temperature (cold and heat) and oxidative stress are often
interrelated; these conditions singularly or in combination induce cellular damage (Arun et al., 2011; Prince et al.,
2013; Morina et al., 2015). Ghosh and Xu (2014) reviewed abiotic stress responses in plants and reported that these
responses occur in various organs especially at roots tissues. Under normal growth condition, root absorbs water and
nutrients from the soil and supplies them throughout the plant body, ensuring a cellular homeostasis balance.
However, this cellular homeostasis altered during the stress period when roots tissues are forced to adopt several
structural and functional modifications. Under cold stress a significant amount of reactive oxygen species (ROS) is
produced, such as hydrogen peroxide (H
2
O
2
) and other dangerous derivatives oxygen, which cause progressive
oxidative damage and as consequence cell death (Mohammadian et al., 2012). During cold acclimation, increases of
enzymatic changes were associated with cold tolerance by a significant capture of these harmful molecules. It is
often assumed that in many species, plants actively produce ROS as signalling molecules to control processes
such as programmed cell death (Parent et al., 2008), abiotic stress responses, for example, in Barley (Baek et al.,
2000), in rice (Kim and Thomas, 2011), in maize (
Zeamays
L.) (Lukatkin, 2002), pathogen defense, in Pepper
(Wang et al., 2013). Higher plants contain numerous enzymatic and non-enzymatic reactive oxygen intermediate
scavengers and antioxidants, both water and lipid soluble, localized in different cellular compartments (Mittler,
2002; Thakur and Nayyar, 2013). Antioxidant enzyme activities in plants are accepted as a good indicator of
tolerance under stress conditions (Öklen et al., 2008).
Lee et al., (2009) argument that roots proteins increases highly under cold stress than in the leaves in rice. On the
other hand, Cavalcanti et al., (2007) investigating the effect of salt stress in cowpea (
Vigna unguiculata
) plants,
concluded that roots and leaves display distinct mechanisms of response during salt stress and recove ry.
