Molecular Plant Breeding 2016, Vol.7, No.12, 1
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Figure 2 Phenotypic distribution of rice grain size related traits, (A) grain length, (B) grain width, (C) grain thickness, (D) length to
width ratio, (E) 1 000-grain weight in the Ce253/NYZ F
2
population. Open arrow and closed arrow indicate the means of Ce253 and
NYZ, respectively. Red curve designates GaussAmp function fitting distribution of frequency
1.3 Genotyping using DNAmolecular markers
The total of 820 SSR and IN/DEL markers were used to detect and screen genetic polymorphism between the two
parents NYZ and Ce253. One hundred and ten polymorphic markers (13.4%), which well-distributed on the whole
genome of rice, were obtained (Table 3; Supplement Table 1). The individuals of F
2
population were genotyped
with these polymorphic markers by PCR. The molecular markers’ genotypes of the population were utilized for
the construction of genetic linkage map. The obtained linkage map spanned 2185.27 cM in length with an average
distance of 19.87 cM between adjacent markers (Table 3).
