MPB-2016v7n12 - page 8

Molecular Plant Breeding 2016, Vol.7, No.12, 1-19
http://mpb.biopublisher.ca
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(P =0.099) (Supplement Figure 1). In F2 population, all five grain size related traits were shown continuous
variation with normal or nearly normal distribution, exhibiting the characteristics of quantitative inheritance
(Figure 2). One or both directions of transgressive segregation presented in all traits except TGW (Figure 2; Table
1).
Figure 1 The comparison of grain size related traits of the two parents, Ce253 and NYZ
Note: The grain size of Ce253 (A) and NYZ (B), and the significant difference between of grain length (C), grain width (D), grain
thickness (E), and 1000-grain weight (F) of two parents. Error bar represent means±s.d. and ** means P≤0.01 in student T-test; Scale
bar, 10mm in (A) and (B)
Table 1 Performance of grain size related traits of the parents and their F2 population
Traits
Parents F2 population
Ce253 NYZ Range Mean SD Kurtosis Skewness
GL (mm)
GW (mm)
GT (mm)
LWR
TGW (g)
10.96
2.76
1.90
3.97
24.91
13.65
3.71
2.43
3.68
50.16
10.40 ～13.49
2.64～3.76
1.7～2.38
2.96～4.78
25.66 ～48.78
11.93
3.17
2.13
3.78
34.39
0.58
0.25
0.09
0.35
4.28
-0.04
-0.36
1.56
-0.11
0.73
0.14
0.22
-0.43
0.16
0.35
1.2 Correlations among five rice grain size related traits
To study the pair-wise correlation relationships between rice grain size related traits, we performed correlation
coefficient analysis using the data obtained from phenotypic evaluation. On the whole, GL and GW showed
significant correlations with LWR. GL was positive correlated with LWR (R 2=0.507, P<0.01), whereas, GW was
negative correlated with LWR (R 2=-0.843, P<0.01). GL, GW, and GT also showed significant positive
correlations with TGW as expected (Table 2). The results indicated that GL, GW, and GT are important for the
formation of TGW.
Table 2 Coefficients of pairwise correlation among the grain size related traits in Ce253/NYZ F2 population
GL GW GT LWR TGW
GL 1
GW 0.026 1
GT
LWR
-0.022
0.507**
-0.034
-0.843**
1
0.020 1
TGW 0.414** 0.414** 0.216** -0.131 1
Note: ** Significance at P≤0.01 levels