Basic HTML Version
International Journal of Marine Science 2013, Vol.3, No.17, 135-144
http://ijms.sophiapublisher.com
143
Figure 5 Changes in lipid:protein ratio within cell of
Dunaliella tertiolecta, Scenedesmus sp
and
Nannochloropsis sp
in response to
varying temperature and nutrient conditions
Note: For data sets, each point is the mean (±SE) of three FTIR spectra from pooled samples (derived from three replicate flasks).
Each of the spectra was separately derived from randomly selected small groups of cells within the vacuum-dried preparation
4 Conclusions
The variation of parameters tested (temperature and
nutrient limitation) strongly influenced the lipid
content of microalgae. All the stress condition
investigated led not only to the accumulation of lipids,
but also to a reduction in microalgae growth, thereby
affecting the lipids productivity.
This study also has shown that
Dunaliella tertiolecta,
Scenedesmus
sp. and
Nannochloropsis
sp. can respond
and distinctive changes in growth and lipid content
with temperature and nutrient stress conditions. These
study also demonstrated that Nile Red staining and
FTIR analysis can effectively identify change in lipid
content in algal cells in response to temperature and
nutrient treatments. This demonstrated that FTIR and
Nile Red will be efficient tool for rapidly monitoring
lipid accumulation of microalgae and will have
applications for algal biofuel production processes.
Acknowledgement
This research was funded by Academic Recharging Program 2010 of
Directorate General of Higher Education, National Education Ministry,
Republic of Indonesia and collaboration with School of Biological Science,
Monash University, Australia. We would like to thank to Prof. John Beardall,
John Arvanitaksis and Amanda Pantorno for their support during
experiments.
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