International Journal of Aquaculture, 2017, Vol.7, No.21, 134-138
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especially on the chain of sea kelp trading system, it is needs attention so that the price at the farm level which is
very volatile can be stabilized. Seaweed can be addressed as well as incentives for good quality seaweed needs to
be contemplated to maintain the quality of seaweed in the future.
Some problems faced for social and economic aspects that influence economic seaweed activity:
(1) The weakness of institutions on the on-farm, access to sources of innovation, market information, market
guarantees, capital and partnership are still weak.
(2) The limited capital for business development.
(3) Human resources who manage the business is still very minimal in terms of both knowledge and skills.
(4) The lack of net-works and partnerships between farmers, processors, entrepreneurs/private and government.
(5) The lack of facilities and infrastructure for the smooth production and marketing of refined products produced.
The number of these problems led to the need for a vehicle in the form of a center of excellence so that the
settlement of the problems associated with the cultivation of seaweed of provision of quality seeds of seaweed,
repairs enlargement techniques, pest and disease management and the environment to the handling of post-harvest
to increase the added value high in the form of processed products or prototypes of products for industrial as well
as social and economic problems. These problems can be solved by conducted in thorough and comprehensive
that consider various factors faced in overcome these problems. The main objective of the study is to know the
effect of the epiphytic and ice-ice diseases in seaweed
Kappaficus alvarezii
, on the growth and carrageenan
content.
Results of the studies will be carried out on the public dissemination or “stakeholders”, so it can benefit the
community and enhance the nation’s competitiveness.
2 Materials and Methods
2.1 Site and time
The seaweed
Kappaphycus alvarezii
sample was collected from Libukang Village, Jeneponto District, South
Sulawesi Province, Indonesia from July-November 2014. The observation of ice-ice and epiphytes diseases were
done in the Laboratory of Parasitic Fish Diseases Department of Fisheries Faculty of Marine Science and
Fisheries of Hasanuddin University, Makassar, Indonesia.
2.2 Sample collection
The infected thallus weretaken randomly for the samples. The thallus were put into a sterile plastic bags and kept
on a ice box (4°C) and then transported to laboratory for further analysis.
2.3 Carrageenan extraction
Extraction of carrageenan was done using the alkali and acid treatment method (Santaley, 1987; Ohno et al., 1994).
Dried materials (50 g) were washed with tap water to remove salt, and then dried at room temperature for 3 h. The
materials were incubated in 2 L 6% KOH (alkali treatment) followed by washing in tap water for 1 h. Another set
of samples were kept in 2 L 5% H
2
SO
4
(pH 3.0) (acid treatment) at 30°C for 3 h. This was followed by washing
for one hour in tap water, then boiled for at least 1 hour and the carrageenan extract was filtered under vacuum.
Persentage of carrageenan content seaweed can be count, using the Formula of Boot (1975, in Gimaruddin, 2006)
as follows:
2.4 Growth rate measurement
The specific growth rate (SGR) data were taken every 10 days interval for 40 days, while the content of
carrageenan rendement was taken at the end of the study. The SGR was calculated based on Poncomulyo et al.
(2006) as follow:
