Field Crop 2018, Vol.1, No.4, 15-17
17
(1) Multiplication of TGMS lines
(2) Hybrid rice seed production
3.1 Multiplication of TGMS lines
Nucleus seed production of a TGMS line starts within the fertility-inducing environment. Seeding of TGMS lines
is organized in such the simplest way that the sensitive stage occurs when the temperature is good for a higher
seed set.
At the duration of flowering, approximately hundred typical plants are chosen from the population of a TGMS
line and their panicles are bagged. The selection method should be done within 1 week.
After the harvest, the chosen plants are scored for spikelet fertility (based on the main panicle) and 50 plants with
higher spikelet fertility (> 30%) are selected.
Progenies of the chosen plants are grown within the sterility-inducing atmosphere. About thirty seeds are taken
from every of the chosen plants to grow single-row progenies and the remaining seeds are put away carefully. The
balance of the seeds of the progenies that are uniform and completely male sterile must be distinct and bulked to
create the nucleus seed.
Nucleus seed of the TGMS line is utilized for producing breeder seed under strict isolation. Breeder seed for the
TGMS line is produced in the fertility-inducing environment.
The breeder seed produced under the oversight of the plant breeder has high genetic purity and utilized for
producing foundation seed of parental lines, which will be used for producing hybrid seed.
3.2 Hybrid rice seed production
In two-line hybrid seed production, the two safe-periods refers to the useful atmospheric conditions that support
first the induction of absolute male sterility and second the facilitation of proper pollen movement from the pollen
parent and fertilization of the TGMS parent (
. So, the first
safe-period determines seed purity, while the second safe-period determines hybrid seed yield (Virmani et al.,
2003).
A multidisciplinary approach in developing superior TGMS lines and pollen parents can help to develop two-line
rice hybrids suitable for the distinct ecological situations in which rice is grown (Kushwaha, 2016). Despite the
promise that two-line hybrid rice technology holds, it would be wise to have a harmonious balance in using
three-line and two line hybrids and conventional rice varieties in an appropriate manner in national rice production
programs.
Authors’ contributions
VM wrote and shaped the manuscript, coauthors help to correct whole document. All authors read and approved the final manuscript.
Acknowledgments
I am thankful to Ujjawal Kr. S. Kushwaha for his critical comments to shape this manuscript well.
Reference
Guidelines for seed production of hybrid rice, 2018
Kushwaha U.K.S., 2016, Rice in Black Rice, Research, History and Development, pp.192
PMid:27051207
PMCid:PMC4819371
Sindhu J.S., and Kumar I., 2018, Quality seed production in hybrid rice
Virmani S.S., Sun Z.X., Mou T.M., Jauhar A.A., and Mao C.X., 2003, Two-line hybrid rice breeding manual, Los Baños (Philippines): International Rice
Research Institute, pp.88
Yuan L.P., 1990, Progress of two-line system hybrid rice breeding, Scientific Agriculture Sin, 3: 1-6
