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Computational Molecular Biology 2015, Vol. 5, No. 1, 1-13
http://cmb.biopublisher.ca
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hemoglobin protected against the mutagenic agents,
thus the most unique efficiencies of hemoglobin in the
camels or other species is related to structure,
composition and features of alpha HBA and HBB due
to the fact that alpha and beta subunit is highly
conserved in camel, cow, horse and human. As well as
illustrated on Figure 3 each chains of hemoglobin only
have one domain (domain 1) in the present study.
Figure 1 Multiple sequence alignment of HB subunits. 1A) α and ß chains of hemoglobin were aligned between human, domestic
one and two-humped camel, wild two humped camel, cow and horse. 1B) Α chain of hemoglobin was aligned between mentioned
species. 1C) B chain of hemoglobin was aligned between mentioned species. Identical gaps and conserved sequences are indicated by
dashes (-) and pink columns respectively
The most frequencies of AAs with consensus
sequences belonged to L-K-V-K-E-G-E-A-L-R-P-T-F-
F-D-L-S-V-K-H-G-K-V-H-L-D-L-L-S-L-H-K-L-V-D-
P-N-F-L-L-L-A-F-T-P-A-K-V-L-Y (Figure 1) that
these are most common modes could be possible.
Based on the consensus sequence can identified the
nucleotides that are less affected by the mutations. For
instance according to Figure 1, in the part of alpha
subunit of hemoglobin in cow the AA (amino acids)
sequences of A-A-G-A-W-V-S-G-A-A-A were
substituted by S-K-T-T-F-I-G-A-K-G-D in the one
humped camel respectively. As well as, in the part of
alpha subunit of hemoglobin in the
Camelus
bactrianus
and
Camelus dromedarius
, amino acids
sequences of S-K-T-F-I-G-A-G were substituted by
P-A-A-W-V-A-G-S in Homo sapiens respectively,
which is also showed in the results of the phylogenetic
diagram (Figure 2). Substitution of amino acid was
not found between
Camelus
family for both subunits
but it was obviously found for other species (Figure 1).