 
          
            Bioscience Method 2018, Vol.9, No.2, 12-21
          
        
        
        
          13
        
        
          Nigeria, there have been limited studies on the utilization of fish as fish based snack (suya). Plants have been
        
        
          reported to have beneficial effects on human health especially in managing and preventing non communicable
        
        
          diseases such as diabetes, obesity, coronary heart diseases etc. Plant crude extracts have the advantage to have
        
        
          been consumed by humans for thousands of years with little or no adverse effect, and besides antimicrobial
        
        
          importance, several plants are being used in different areas of human health such as traditional medicine,
        
        
          functional foods, dietary supplements and recombinant protein manufacturing (Carmen et al., 2014) Their role as
        
        
          antimicrobials derives from their capacity to control natural spoilage processes (food preservation) and to
        
        
          prevent/control growth of microorganisms, including pathogenic micro-organisms (Tajkarimi et al., 2012).
        
        
          Apart from the use of herbs for antimicrobial purposes, herbs and spices are also of medicinal importance to
        
        
          human health. Bitter leaf (
        
        
          
            Vernonia amygdalina
          
        
        
          ) and scent leaf (
        
        
          
            Ocimum gratissimum
          
        
        
          ) as herbs have been
        
        
          reported by many researchers to be highly medicinal.
        
        
          
            Vernonia amygdalina
          
        
        
          has been found useful in the ethno
        
        
          therapy of asthma, schistosomiasis, malaria, measles, diarrhoea, tuberculosis, abdominal pain and fever. Arhoghro
        
        
          et al. (2009), reported that
        
        
          
            O. gratissimum
          
        
        
          crude extracts significantly reduced and even reversed carbon
        
        
          tetrachloride (CCl
        
        
          4
        
        
          )-induced liver damage in Wister rats after establishment. Hence
        
        
          
            O. gratissimum
          
        
        
          might be an
        
        
          effective hepatoprotector in the diet of patients with hepatopathies (Arhoghro et al., 2009). Spices (Ginger, Garlic,
        
        
          African negro pepper, Peanuts) have been reported as a remedy for various ailments such as arthritis, muscle
        
        
          soreness, chest pain, low back pain, stomach pain, and menstrual pain, dyspepsia, acute or chronic gastritis,
        
        
          diarrhea, cardiovascular disease, arterial sclerosis and other disease related to oxidative stress (Manach et al.,
        
        
          2005). It can be used for treating upper respiratory tract infections, cough, and bronchitis. As an anti-inflammatory
        
        
          agent, it is recommended for joint problems (Shukla and Singh, 2007).
        
        
          To gain consumer’s acceptance of a fish based product rather than the carbohydrate snacks, the consumer would
        
        
          have to be convinced of the quality and possible nutritional advantages with respect to the standard product it is
        
        
          replacing. This study examines the effect of plants (
        
        
          
            Ocimum gratissimum
          
        
        
          and
        
        
          
            Vernonia amygdalina
          
        
        
          ) crude extract
        
        
          on the nutritional quality of
        
        
          
            Sarotherodon galilaeus
          
        
        
          snack.
        
        
          
            1 Materials and Methods
          
        
        
          
            1.1 Source of raw materials
          
        
        
          The fish sample used for this study
        
        
          
            (Sarotherodon galilaeus)
          
        
        
          were procured from fish mongers at the landing site
        
        
          of the fishermen at Egbe reservoir, Ode-Ekiti, Ekiti State, South west Nigeria. Nine hundred and forty grams of
        
        
          
            Vernonia amygdalina
          
        
        
          and 827 gms of
        
        
          
            Ocimum gratissimum
          
        
        
          leaves and ingredients for the composite spice (suya
        
        
          spice) and other materials used were procured from Akungba Akoko, Ondo state, Nigeria.
        
        
          
            1.2 Production of spices
          
        
        
          Table 1 shows the proportions and grams of different ingredients used in the spice. The following ingredients are;
        
        
          Human edible Groundnut cake locally called “kulikuli”, Ginger, Garlic, Cayenne pepper flakes, Black Pepper,
        
        
          Cooking salt and African Negro pepper. These ingredients were ground into powdery form to increase surface area
        
        
          and improve attachment. The spice was similar to “Yaji” that is “suya” spice.
        
        
          Table 1 Spice composition
        
        
          Ingredients
        
        
          Grams of ingredient
        
        
          Proportion in spice (%)
        
        
          Edible Groundnut Cake
        
        
          765
        
        
          85
        
        
          Ginger
        
        
          40.5
        
        
          4.5
        
        
          Garlic
        
        
          30.6
        
        
          3.4
        
        
          Cayenne Pepper Flake
        
        
          30.6
        
        
          3.4
        
        
          Black Pepper
        
        
          17.1
        
        
          1.9
        
        
          Common Salt
        
        
          15.3
        
        
          1.7
        
        
          African Negro Pepper
        
        
          0.9
        
        
          0.1
        
        
          Total
        
        
          900
        
        
          100