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Bioscience Methods 2015, Vol.6, No.1, 1-13
http://bm.biopublisher.ca
1
Research Article Open Access
Eleven
StOSMs
Genes in the Potato Genome Response to Water Deficiency
Linbo Qiao, Huihua Fan, Xiaoping Zhang, Haixia Dai, Xinling Yao
The State Education Ministry Laboratory of West Bioresource Protection and Utilization, Life Science College, Ningxia University, Yinchuan, Ningxia 750021,
China
Corresponding author email: Email
Bioscience Methods, 2015, Vol.6, No.1 doi: 10.5376/bm.2015.06.0001
Received: 08 Nov., 2014
Accepted: 13 Jan., 2015
Published: 20 Jan., 2015
Copyright
©
2015 Qiao et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Qiao et al., 2015, Eleven
StOSMs
Genes in the Potato Genome Response to Water Deficiency, Bioscience Methods, Vol.6, No.1 1
-
13 (doi:
Abstract
Osmotin plays an important role in plant response to low temperature and pathogen infection. To date little is known on
existence and full function of
OSM
genes in genome-wide. In this study, based on an
OSM
expression sequence tag identified from a
drought-treated subtractive library,
StOSMs
in the potato genome were identified and characterized in response to a water deficit
gradient. BLAST and bioinformatic analysis revealed that there are at least eleven
StOSMs
in the potato genome. Among eleven
StOSMs
,
eight
StOSM
mRNAs accumulated in leaf at the drought-lethal critical point (DLP) were at least 4-fold higher than in the
control. The peak
StOSM-
8E mRNA accumulation was 49-fold higher than the control. Three
StOSM
mRNAs in leaf at DLP were
9.2-fold lower than in the control. This result of qRT-PCR quantification was identical with the FPKM values of all eleven
StOSMs
examined in the the Potato Genome Sequence database (PGSD). In conclusion, water deficiency does induce the regulation of the
expression of the eleven
StOSMs.
Either up or downregulated by water deficiency,
StOSM
s play a positive role in enhancing potato
tolerance to drought stress. Therefore, osmotins can be considered to be drought responsive molecules involved in withstanding
water deficits. The result provides an evidence to reveal how potato adapts to drought stress.
Keywords
OSM; Osmotin; Expression; mRNA; Drought
Introduction
Osmotin
(accession No. M29279), encoding the stress
response molecule osmotin, was initially identified in
cultured tobacco cells (
Nicotiana tabacum
L. cv
Wisconsin 38) induced by low water potential (Singh
NK et al 1989). Subsequently, it was showed that
abscisic acid (ABA), low temperature and NaCl can
induce the accumulation of three
StOSM
mRNAs
(pA13, pA35, and pA81) in
S. commersonii
grown
in
vitro
(Zhu et al 1995). Later on
StOSM
was
shown to
exhibit antifungal activity against a range of fungal
pathogens (Rivero et al. 2012, Mani et al. 2012 and
Patade et al. 2013).
Recently,
StOSM
overexpression
in transgenic Arabidopsis and pepper results in H
2
O
2
accumulation and the response of hypersensitive cell
death in the leaf and reduces susceptibility to pathogen
infection (Choi
et al. 2013).
Remarkably, it is showed that plant osmotin, by
binding to the adiponectin receptor as an agonist,
activates a pathway concerned with the resistance to
animal disease. Osmotin reduces ethanol neurotoxicity
via the upregulation of the antiapoptotic Bcl-2 protein,
and reverses synaptic dysfunction and neuronal
apoptosis. The Bcl-2 protein appears to block a distal
step in a common pathway for apoptosis and
programmed cell death (Naseer et al. 2014 and Shah et
al. 2014).
Osmotin is not only an intercellular component
involved in stress tolerance but also an agonist that
alternatively binds to the adiponectin receptor
involved in animal apoptosis. However, although
osmotin was identified in tobacco in 1989, little is
known regarding the details of plant osmotin,
particularly how many
OSM
members are present in a
plant genome and how these members work together
in response to stress.
In potato, nine
StOSM
genes were identified by
screening a bacterial artificial chromosome (BAC)
library. The nine
StOSM
genes are organised into two
loci on chromosomes 08 and 11 (Castillo et al. 2005).
Whether there are additional
StOSM
genes in the
potato genome and the functional differences among
the potato
StOSM
genes regarding the response to
abiotic stress, such as drought, remain unknown.