Page 11 - Plant Gene and Trait

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Evolutionary studies in sub-families of Leguminosae family based on
matK
gene
8
(Papilionaceae), Mimosaceae and Caesalpiniaceae;
This study shows that based on morphological
characters species of each subfamily are classified
differently and they fall in different groups based on
DNA and Protein sequences of matK. In this study we
observed that as per the botanical classification species
are classified differently based on their morphological
features like species’ flower color, size and shape,
types and arrangements of Stipules, size of plant etc.
But this study focus on evolutionary relationship of
species in the Leguminosae Family species based on
DNA and Protein sequences of
matK
gene with
Multiple sequence alignment by Maximum likelihood
and Neighbor Joining methods where we observed that
some species belonging to same genus are fall very
nearly as per botanical classification which is correct as
per both botanical and evolutionary relationship but we
observed that few species are distantly related even if
they are from same genus. Even some species’ position
also remains unchanged in both Maximum likelihood
and Neighbor Joining methods.
Acknowledgement
We are heartily thankful to Prof. (Dr.) P.V. Virparia, Director,
GDCST, Sardar Patel University, Vallabh Vidyanagar, for
providing us facilities for the research work.
References
Cameron K. M.. 2005. Leave it to the leaves: a molecular
phylogenetic study of Malaxideae (Orchidaceae).
American Journal of Botany 92: 1025-1032.
http://dx.doi.org/10.3732/ajb.92.6.1025
Ems S. C. Morden C. W. Dixon C. K. Wolfe K. H. dePamphilis
C. W. Palmer J. D.. 1995. Transcription, splicing and
editing of plastid RNAs in the nonphotosynthetic plant
Epifagus virginiana. Plant Molecular Biology 29: 721-733.
http://dx.doi.org/10.1007/BF00041163
Gadek, P. A., P. G. Wilson, AND C. J. Quinn. In press
Phylogenetic reconstruction in Myrtaceae using matK, with
particular reference to the position of Psiloxylon and
Heteropyxis. Australian Systematic Botany.
Harborne, J.B. 1994. Phytochemistry of the Leguminosae. In
Phytochemical Dictionary of the Leguminosae, eds
Bisby,F.A. et al. London: Chapman & Hall
Hayashi K. Kawano S. 2000. Molecular systematics of Lilium
and allied genera (Liliaceae): phylogenetic relationships
among Lilium and related genera based on the rbcL and
matK gene sequence data. Plant Species Biology 15: 73-93.
http://dx.doi.org/10.1046/j.1442-1984.2000.00025.x
Hilu K. W. Borsch T. Müller K. Soltis D. E. Soltis P. S.
Savolainen V. Chase M. W. Powell M. P. Alice L. A. Evans
R. Sauquet H. Neinhuis C. Slotta T. A. B. Jens G. R.
Campbell C. S. Chatrou L. W. 2003. Angiosperm
phylogeny based on matK sequence information. American
Journal of Botany 90: 1758-1776.
http://dx.doi.org/10.3732/ajb.90.12.1758
Hilu KW, Liang H: The matK gene: sequence variation and
application in plant systematics. American Journal of
Botany 1997, 84:830-839.
http://dx.doi.org/10.2307/2445819
Johnson L. A. Soltis D. E. 1994. matK DNA sequences and
phylogenetic reconstruction in Saxifragaceae s. str.
Systematic Botany 19: 143-156.
http://dx.doi.org/10.2307/2419718
Martin F. Wojciechowski,matt Lavin,michael J. Sanderson. A
Phylogeny Of Legumes (Leguminosae) Based On Analysis
Of The Plastid Matk Gene Resolves Many Well-supported
Subclades Within The Family.
Michelle M. Barthet, Hilu KW : Expression of matK:
functional and evolutionary implications. American
Journal of Botany 2007, vol. 94 no. 8 1402-1412.
http://dx.doi.org/10.3732/ajb.94.8.1402
Mohr G. Perlman P. S. Lambowitz A. M. 1993. Evolutionary
relationships among group II intron-encoded proteins and
identification of a conserved domain that may be related to
maturase function. Nucleic Acids Research 21: 4991-4997.
http://dx.doi.org/10.1093/nar/21.22.4991
Neuhaus H. Link G. 1987. The chloroplast tRNALys (UUU)
gene from mustard (Sinapsis alba) contains a class II intron
potentially coding for a maturase-related polypeptide.
Current Genetics 11: 251-257.
http://dx.doi.org/10.1007/BF00355398
Patel SS, Vaidya MB, Shah DB (2014) Homology Modelling of
Conserved rbcL Amino Acid Sequences in Leguminosae
Family. J Data Mining Genomics Proteomics 5: 154.
doi:10.4172/2153-0602.1000154.
Phylogenetic and molecular evolutionary analyses were
conducted using MEGA version 5 (Tamura, Peterson,
Stecher, Nei, and Kumar 2011).
Polhill, R.M. & Raven, P.H. (eds) 1981. Advances in Legume
Systematics. Royal Botanic Gardens, Kew
Sagar Patel and Shah, 2014, Phylogeny in Few Species of
Leguminosae Family Based on matK Sequence,
Computational Molecular Biology, Vol.4, No.6 1-5
(doi:10.5376/cmb.2014.04.0006).
http://dx.doi.org/10.5376/cmb.2014.04.0006
Sagar Patel, and Hetalkumar Panchal. 2014, Bioinformatics
Information of Leguminosae Family in Gujarat State,
International Journal of Agriculture, Environment &
Biotechnology: Vol 7, Issue 1, Pages:11-15.
Sagar Patel, Hetalkumar Panchal. Evolutionary studies of few
species belonging to Leguminosae family based on RBCL
gene. Discovery, Volume 9, Number 22, January 2014,
Pages: 38-50. ISSN 2278 – 5469, EISSN 2278 – 5450.
Sagar Patel, Panchal H., 2013. Leguminobase: A Tool To Get
PLANT GENE AND TRAIT