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sequential order remains unchanged in both results.

So,there might be strong sequence similarity between

them, and the results remain unchanged in two

different methods.

2.3 Fabaceae Family

As shown in above Figures 8 and 8.1, species of

Medicago, Sesbania, Butea, Lathyrus, Vicia,

Canavalia, Crotalaria

and few species of

Vigna

and

Trigonella

are related as per morphological characters

or botanical classifications except

Tephrosia purpurea

and

Vigna unguiculata

which are distantly related to

other species of their respective genera. Most of genera

shown accurate result of their species compared with

their botanical classification and evolutionary results

which is discussed above and match correctly with

each other.

Figure 8. Result by Max.Likelihood Bootstrap Method

Figure 8.1 Result by NJ Bootstrap Method

The results of bootstrap by Maximum Likelihood and

NJ methods showed striking similarity in the

phylogenetic relationship among the species. This is an

indication of similar sequence similarity among the

species

.

[IV] Conclusion

Reconstructing the phylogenetic relationships of the

Leguminosae is essential for understanding the origin

and diversification of this ecologically and

economically important family of angiosperms. Strong

phylogenetic signal from

matK

has rendered it an

invaluable gene in plant systematics and evolutionary

studies at various evolutionary depths. Species

belonging to Leguminosae family which is further

divided into three subfamilies like Fabaceae

LablabPurpureus

VignaVexillata

VignaAconitifolia

VignaUnguiculata

DolichosTrilobus

VignaTrilobata

VignaUmbellata

PhaseolusVulgaris

VignaAngularis

VignaRadiata

AlysicarpusVaginalis

GlycineMax

PuerariaLobata

CajanusCajan

ButeaMonosperma

ButeaSuperba

AbrusPrecatorius

CanavaliaEnsiformis

CanavaliaGladiata

ClitoriaTernatea

IndigoferaTinctoria

GliricidiaSepium

SesbaniaCannabina

SesbaniaBispinosa

SesbaniaGrandiflora

SesbaniaSesban

AeschynomeneIndica

DalbergiaSissoo

ArachisHypogaea

PterocarpusMarsupium

Crotalaria juncea

CrotalariaSpectabilis

AlhagiPseudalhagi

TavernieraCuneifolia

ViciaHirsuta

ViciaSativa

TrigonellaFoenum‐graecum

MedicagoLupulina

MedicagoPolymorpha

MedicagoLaciniata

MedicagoSativa

TrigonellaCorniculata

CicerArietinum

MelilotusAlbus

TrifoliumRepens

LathyrusSativus

LathyrusAphaca

PisumSativum

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