Molecular Pathogens, 2025, Vol.16, No.6, 276-284 http://microbescipublisher.com/index.php/mp 281 Figure 2 Response curves between bacterial growth and (a) suspension electrical conductivity (EC) and (b) suspension pH in the community tolerance assays. Depicted are representative examples of response curves of soils of different soil EC and soil pH. (c) Relationship between soil EC and community-level salt tolerance (indicated by IC50) along both salinity gradients (AG and NV gradient). (d) Relationship between soil pH and community-level pH tolerance (indicated by pHopt). Samples with a pH < 5.5 (open symbols) were excluded from the regression analysis (Adopted from Rath et al., 2018) 7 Conclusions In recent years, many field experiments have successively pointed out that merely improving varieties is far from enough on saline-alkali land. The combined use of microbial "helpers" such as plant rooting bacteria (PGPR) and arbusyclal mycorrhizal fungi (AMF), along with organic materials like compost and biochar, can indeed significantly improve the performance of corn - not only does it grow better, but the yield also increases and its resistance becomes stronger. Especially in terms of soil fertility, salinity relief, nutrient utilization and microbial community diversity, the effects are more obvious. Some methods, such as simultaneous inoculation of PGPR and AMF, or microcapsule seed coating and nano-spray treatment, have also been proven effective in practice (although they are still in the stage of continuous exploration). These combined solutions not only address the salt issue but also gradually bring the soil system itself to life. However, this road is not without obstacles. The complexity of the problem lies in the fact that different types of soil, varying degrees of salinization, and the genotypes of corn itself do not provide uniform feedback on microbial preparations. Sometimes, what works in one environment may not work in another. Moreover, most studies are still at the stage of short-term observation and lack data support from long-term tracking and large-scale trials. If it is to be truly implemented, relying solely on laboratories and small plots of land is not enough. To clarify the intricate relationships among corn, microorganisms and environmental stress, systematic research is still needed.
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