Molecular Pathogens, 2025, Vol.16, No.5, 246-256 http://microbescipublisher.com/index.php/mp 254 understanding of the components of wheat root exudates, but there is still a lack of identification and functional verification of many unknown compounds. In the future, it is necessary to use high-resolution mass spectrometry, nuclear magnetic resonance and other analytical methods to construct a spectrum library of wheat root exudates, and to clarify the role of key compounds in growth promotion, disease resistance and other interactions through bioassays and genomics. The diffusion, transformation and utilization of root exudates in the rhizosphere microenvironment are dynamic processes that are difficult to capture in situ using traditional methods. In the future, microelectrode sensing technology and isotope tracing technology can be combined to achieve real-time monitoring of secretion release and microbial response from a single root at a single moment. Multi-omics integrated research, root exudates-microbe interactions involve the interaction between plant metabolism and microbiota metabolism. Existing research on the adaptability of different environments and varieties is mostly conducted under controlled conditions or limited varieties, and lacks universal verification in different soil types, climate zones and wheat varieties. In the future, large-scale field experiments and diversity analysis should be carried out to understand the consistency and differences of root exudate interaction mechanisms under various scenarios. Acknowledgments The successful completion of this study cannot be separated from the support of the team and the suggestions for the revision of peer review. We would like to express my sincere thanks. Conflict of Interest Disclosure The authors confirm that the study was conducted without any commercial or financial relationships and could be interpreted as a potential conflict of interest. References Afridi M., Kumar A., Javed M., Dubey A., De Medeiros F., and Santoyo G., 2023, Harnessing root exudates for plant microbiome engineering and stress resistance in plants, Microbiological Research, 279: 127564. https://doi.org/10.1016/j.micres.2023.127564 Agnihotri R., Sharma M.P., Prakash A.K., Ramesh A., Bhattacharjya S., Patra A., Manna M.C., Kurganova I., and Kuzyakov Y., 2021, Glycoproteins of arbuscular mycorrhiza for soil carbon sequestration: review of mechanisms and controls, The Science of the Total Environment, 806: 150571. https://doi.org/10.1016/j.scitotenv.2021.150571 Álvarez Y., Otero P., Prieto M.A., Simal-Gándara J., Reigosa M.J., Sánchez-Moreiras A., and Hussain M., 2023, Testing the role of allelochemicals in different wheat cultivars to sustainably manage weeds, Pest Management Science, 79(7): 2625-2638. https://doi.org/10.1002/ps.7444 Ateş Ö., and Kıvanç M., 2020, Solation of acc deaminase producing rhizobacteria from wheat rhizosphere and determinating of plant growth activities under salt stress conditions, Applied Ecology and Environmental Research, 18(4): 5997-6008. https://doi.org/10.15666/aeer/1804_59976008 Bardelli T., Fornasier F., Novarina E., Donniacuo A., Romano E., Bianchi P.G., and Giulini A.P.M., 2024, Changes in the rhizosphere biome depending on the variety of wheat timing of its growing season and agrochemical components in the soils of Italy, Agronomy, 14(4): 832. https://doi.org/10.3390/agronomy14040832 Chen S., Waghmode T.R., Sun R., Kuramae E.E., Hu C., and Liu B., 2019, Root-associated microbiomes of wheat under the combined effect of plant development and nitrogen fertilization, Microbiome, 7(1): 136. https://doi.org/10.1186/s40168-019-0750-2 Cherif-Silini H., Thissera B., Bouket A.C., Saadaoui N., Silini A., Eshelli M., Alenezi F.N., Vallat A., Luptakova L., Yahiaoui B., Cherrad S., Vacher S., Rateb M., and Belbahri L., 2019, Durum wheat stress tolerance induced by endophyte Pantoea agglomerans with genes contributing to plant functions and secondary metabolite arsenal, International Journal of Molecular Sciences, 20(16): 3989. https://doi.org/10.3390/ijms20163989 Cho G., Kim D.R., and Kwak Y.S., 2024, Ecological shifts in soil microbiota and root rot disease progress during ginseng monoculture, Frontiers in Microbiology, 15: 1442208. https://doi.org/10.3389/fmicb.2024.1442208 De Souza Campos P.M., Meier S., Morales A., Borie F., Cornejo P., Ruíz A., and Seguel A., 2022, Root traits distinguish phosphorus acquisition of two wheat cultivars growing in phosphorus-deficient acid soil, Rhizosphere, 22: 100549. https://doi.org/10.1016/j.rhisph.2022.100549 De Werra P., Pechy-Tarr M., Keel C., and Maurhofer M., 2009, Role of gluconic acid production in the regulation of biocontrol traits of Pseudomonas fluorescens CHA0, Applied and Environmental Microbiology, 75: 4162-4174. https://doi.org/10.1128/aem.00295-09
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