Molecular Pathogens, 2025, Vol.16, No.4, 193-206 http://microbescipublisher.com/index.php/mp 204 multiple diseases is a topic of practical significance. At the same time, the principles of hormone interaction can also be learned in cultivation management, such as spraying plant immune-induced anti-agents (partially imitating SA effects) during the high incidence of diseases to improve resistance, while spraying JA inducers or ET release agents during the pest period to ultimately achieve "precise anti-agents". Apply the microbiome to improve cucumber disease resistance. Rhizosphere and leaf microorganisms are closely related to plant immunity. Crowning beneficial microorganisms can activate the ISR pathway, which is closely related to JA/ET signaling. Comprehensive disease-resistant breeding and cultivation management strategies. Disease-resistant varieties are not omnipotent, and they may still occur in high-pressure bacterial sources. Therefore, we must adhere to the principles of comprehensive prevention and control, such as rotation of crops to reduce soil-borne bacterial sources, optimize dense planting and pruning to reduce high humidity, and use insect prevention networks to prevent virus transmission vectors. Only by combining these agricultural measures with disease-resistant varieties can these optimal disease prevention and control effects be achieved. Acknowledgements We thank the anonymous reviewer for their valuable comments that will help improve the quality of this manuscript. At the same time, thank you to colleagues and technicians who provided support and assistance during this research process. Conflict of Interest Disclosure The authors confirm that the study was conducted without any commercial or financial relationships and could be interpreted as a potential conflict of interest. References Akhtar J., Gautam D., Nath R., Gaikwad A.B., Bhat K.V., Mondal B., Jat G., Iquebal A., Tiwari B., and Archak S., 2020, Identification of new resistant sources against downy mildew disease from a selected set of cucumber germplasm and its wild relatives, Indian Journal of Genetics and Plant Breeding, 80(04): 427-431. https://doi.org/10.31742/ijgpb.80.4.8 Amin B., Atif M.J., Pan Y., Rather S.A., Ali M., Li S., and Cheng Z., 2023, Transcriptomic analysis of Cucumis sativus uncovers putative genes related to hormone signaling under low temperature (LT) and high humidity (HH) stress, Plant Science, 333: 111750. https://doi.org/10.1016/j.plantsci.2023.111750 Bandamaravuri K.B., Nayak A.K., Bandamaravuri A.S., and Samad A., 2020, Simultaneous detection of downy mildew and powdery mildew pathogens on Cucumis sativus and other cucurbits using duplex-qPCR and HRM analysis, AMB Express, 10(1): 135. https://doi.org/10.1186/s13568-020-01071-x Caarls L., Pieterse C.M.J., and Van Wees S.C.M., 2015, How salicylic acid takes transcriptional control over jasmonic acid signaling, Frontiers in Plant Science, 6: 170. https://doi.org/10.3389/fpls.2015.00170 Chen J., Mohan R., Zhang Y., Li M., Chen H., Palmer I., Chang M., Qi G., Spoel S., Mengiste T., Wang D., Liu F., and Fu Z., 2019, NPR1 promotes its own and target gene expression in plant defense by recruiting CDK81, Plant Physiology, 181: 289-304. https://doi.org/10.1104/pp.19.00124 He X., Guo S., Wang Y., Wang L., Shu S., and Sun J., 2019, Systematic identification and analysis of heat-stress-responsive lncRNAs circRNAs and miRNAs with associated co-expression and ceRNA networks in cucumber (Cucumis sativus L.), Physiologia Plantarum, 168(3): 736-754. https://doi.org/10.1111/ppl.12997 Huang L., Zhang J., Lin Z., Yu P., Lu M., and Li N., 2022, The AP2/ERF transcription factor ORA59 regulates ethylene‐induced phytoalexin synthesis through modulation of an acyltransferase gene expression, Journal of Cellular Physiology, 239(10): e30935. https://doi.org/10.1002/jcp.30935 Huawei L., Laixin L., Pengfei L., Chaoqiong L., and Jianqiang L., 2016, Expression of cucumber green mottle mosaic virus movement protein in cucumber leads to the expression changes of endogenous gene, J. Hortic Sci Res, 1(1): 1-6. https://doi.org/10.36959/745/391 Innark P., Panyanitikoon H., Khanobdee C., Samipak S., and Jantasuriyarat C., 2020, QTL identification for downy mildew resistance in cucumber using genetic linkage map based on SSR markers, Journal of Genetics, 99(1): 81. https://doi.org/10.1007/s12041-020-01242-6 Kęska K., Szcześniak M.W., Adamus A., and Czernicka M., 2021, Waterlogging-stress-responsive LncRNAs their regulatory relationships with miRNAs and target genes in cucumber (Cucumis sativus L.), International Journal of Molecular Sciences, 22(15): 8197. https://doi.org/10.3390/ijms22158197
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