Molecular Pathogens, 2025, Vol.16, No.4, 193-206 http://microbescipublisher.com/index.php/mp 203 Figure 3Csamlo8-cr mutant plants are resistant to powdery mildew (Adopted from Shnaider et al., 2022) Image caption: A, Image of Csamlo8-cr-1 plant (white arrow) and control plant (cultivar Ilan, red arrow) inoculated with Podosphaera xanthii at 10 days postinoculation (DPI). B, Image of Csamlo8-cr-2 plant (white arrow) and control (cultivar Ilan, red arrow) plants inoculated with P. xanthii at 20 DPI. C and D, Leaf images of Csamlo8-cr-1, Csamlo8-cr-2, and Ilan plants at 10 DPI (C) and 20 DPI (D) (Adopted from Shnaider et al., 2022) In addition to knocking out susceptible genes, CRISPR technology can also be used to site-directly modify cis regulatory elements or key sites of disease-resistant genes to enhance their function. The resistance difference of CsChi23 gene that is resistant to blight comes from a SNP polymorphism on the promoter. In the future, we can try to use a base editor to transform this site of the allelle of the disease into a disease-resistant type, so as to accurately introduce resistance into the commercial variety background. Another role of CRISPR technology in cucumber disease resistance research is to accelerate gene function verification. Genetically transgenic often has long cycles and strict supervision, while CRISPR can quickly obtain functional deletion materials of the gene of interest in a non-transgenic context (excluding exogenous Cas9 through the isolation of offspring), and is used to identify its role in disease resistance. 8.3 Future research direction and comprehensive disease prevention and control strategies The formation of cucumber disease resistance is the result of the combined action of multiple mechanisms, including hormone signal regulation, disease-resistant gene function, transcriptional regulatory network, non-coding RNA and epigenetics. First, explore more genes and mechanisms of broad-spectrum resistance. Secondly, strengthen the application research on hormone signal interaction. The environmental conditions and spatial and temporal dynamics of different diseases are different. How to regulate the signal ratios such as SA, JA, ET in the cucumber body according to specific conditions, so as to achieve balanced prevention and control of
RkJQdWJsaXNoZXIy MjQ4ODYzNA==