Molecular Pathogens, 2025, Vol.16, No.4, 193-206 http://microbescipublisher.com/index.php/mp 199 Figure 1 High-temperature resistance analysis of Csspt mutant cucumber (Adopted from Liang et al., 2023) Image caption: A frameshift mutation of the Csspt mutant lines formed by the CRISPR-Cas9 system. The blue rectangles represent exons, black lines indicate introns, red box in the first exon denotes the target region of the CRISPR system. B—R. Phenotypic characteristics (B–E), roots (F–I), roots cell viability (J–M), stems (N–Q), stem cross area (R) of the wild-type (WT) and Csspt mutant plants under normal temperature conditions and 3-days high-temperature stress. S. The heat injury level of the WT and Csspt plants after three days of exposure to high-temperature stress. Scale bars represent 2 cm in (B–I), 200 μm in (J–M), and 1 cm in (N–Q). The error bars in (R) represent the standard deviations of nine biological replicates. Double asterisks (∗∗) indicate significant difference at P < 0.01 (Student's t-test) (Adopted from Liang et al., 2023) 6 Epigenetics and Gene Expression Regulation 6.1 The role of DNA methylation in pathogen response Epigenetic regulation is one of the important factors in the differences in plant disease resistance. DNA methylation, as an epigenetic marker at the genome level, can change gene expression without changing DNA
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