MP_2024v15n4

Molecular Pathogens 2024, Vol.15, No.4, 179-188 http://microbescipublisher.com/index.php/mp 184 5.3 Case study: Puccinia striiformis and wheat interaction The interaction between wheat and Puccinia striiformis f. sp. tritici (Pst) serves as a model for understanding the transcriptomic basis of disease resistance. The identification of Pst effectors and their roles in wheat immune responses has been a focal point of research. For instance, the effector Pst-milR1 suppresses wheat's immune response by targeting the PR2 gene, highlighting the role of cross-kingdom RNA interference in pathogen virulence (Wang et al., 2017). Another effector, PNPi, interacts with wheat NPR1, reducing the expression of PR genes and compromising the plant's defense (Wang et al., 2016). The resistance gene Yr15 in the wheat line Avocet-Yr15 confers immunity to Pst by activating a global anti-oxidative-stress response, which includes genes involved in maintaining cell viability and redox homeostasis (Figure 3) (Seifi et al., 2021). Additionally, the transcription factor TaMYB391 enhances wheat resistance to Pst by inducing HR-associated cell death and PR gene expression (Hawku et al., 2021). These studies provide a comprehensive understanding of the molecular mechanisms underlying wheat's resistance to Pst, emphasizing the roles of effectors, resistance genes, and transcription factors in shaping the transcriptomic landscape during pathogen interaction. Figure 1 Susceptibility and resistance responses to Puccinia striiformis f. sp. tritici isolate UGW16001 in the wheat lines Avocet-S andAvocet-Yr15at 21 dpi (Adopted from Seifi et al., 2021) Photo caption: (a) Top views of infected Avocet-S and Avocet-Yr15 plants. (b) Macroscopic and microscopic appearance of disease symptoms (left) and immunity response (right). Heavy sporulation can be seen in the pustule/uredinia formed on the Avocet-S leaf (left). Fungal structures were stained with trypan blue. Scale bars = 50 µm (Adopted from Seifi et al., 2021)

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