Molecular Microbiology Research 2024, Vol.14, No.6, 259-270 http://microbescipublisher.com/index.php/mmr 263 Figure 4 Symptoms of leaf spot disease 7 d after inoculation of G. jasminoides leaves. Bars, 1 cm Figure 5 Melatonin treatment applied toA. alternataon PDA plates Image caption: a: The colony morphology of A. alternata treated with different concentrations of melatonin after 7 d and 14 d. b, c: The colony diameter 7 d and 14 d, respectively, after treatment. Bars, 2 cm. Significance was determined according to Duncan’s multiple range test at P<0.05. The data (mean ± SD) were calculated using three replicates 2.6 Effects of melatonin treatment onG. jasminoides leaves Infected withA. alternata We also investigated whether melatonin affected the disease resistance of G. jasminoides leaves against A. alternata. We treated detached leaves with different melatonin concentrations before and after inoculation with A. alternata. The lesion areas of both treatments (before and after inoculation) were significantly reduced on melatonin-treated leaves compared to ddH20-treated leaves, and treatment before A. alternata inoculation showed
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