MMR_2024v14n4

Molecular Microbiology Research 2024, Vol.14, No.4, 162-170 http://microbescipublisher.com/index.php/mmr 168 8 Concluding Remarks Historically, fungal and bacterial diseases in rye have posed significant challenges to agriculture, with ergot (Claviceps spp.) being one of the most notorious. Ergot has been a known issue since the early Middle Ages, causing severe health problems due to the toxic alkaloids produced by the fungus. Traditional methods to combat these diseases included crop rotation, selection of resistant varieties, and manual removal of infected plants. However, these methods were often labor-intensive and not entirely effective. In modern times, the approach to managing rye diseases has evolved significantly. Advances in genetic research have led to the identification of resistant genotypes and the development of hybrid varieties with improved resistance to diseases like ergot and rust. Molecular breeding techniques, such as the introgression of effective restorer genes, have shown promise in reducing ergot infection levels. Additionally, fungicide treatments, although limited in efficacy and dependent on weather conditions, have been employed to manage fungal infections. The use of genome-wide association studies (GWAS) has furthered our understanding of the genetic basis of disease resistance. For instance, research on Fusarium culmorum has identified key SNPs associated with aggressiveness and mycotoxin production, providing valuable insights for resistance breeding. Moreover, the discovery of natural products with antimicrobial properties has opened new avenues for developing biocontrol agents. The future of rye disease management lies in the integration of advanced genetic tools and sustainable agricultural practices. Continued research into the genetic diversity of rye and its pathogens will be crucial. For example, the identification of novel resistance genes, such as Pm17 and Pm8, and their evolutionary divergence offers potential for developing new resistant varieties. Additionally, the use of resistant germplasm and the selection of distinct donors for breeding programs will enhance the genetic variability and durability of disease resistance in rye. Biotechnological advancements, such as CRISPR/Cas9, could be leveraged to introduce specific resistance traits into rye cultivars more efficiently. Furthermore, the development of environmentally friendly fungicides and biocontrol agents will play a significant role in sustainable disease management. The integration of these modern approaches with traditional practices, such as crop rotation and proper field management, will provide a holistic strategy to combat fungal and bacterial diseases in rye. Acknowledgments We express our sincere gratitude to two reviewers for their suggestions. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Acharya J., Bakker M., Moorman T., Kaspar T., Lenssen A., and Robertson A., 2018, Effects of fungicide seed treatments and a winter cereal rye cover crop in no till on the seedling disease complex in corn, Canadian Journal of Plant Pathology, 40: 481-497. https://doi.org/10.1080/07060661.2018.1506503 Ashraf R., Johansson E., Vallenback P., Steffenson B., Bajgain P., and Rahmatov M., 2022, Identification of a small translocation from 6R possessing stripe rust resistance to wheat, Plant Disease, 107(3): 720-729. https://doi.org/10.1094/PDIS-07-22-1666-RE Bakker M., Acharya J., Moorman T., Robertson A., and Kaspar T., 2016, The potential for cereal rye cover crops to host corn seedling pathogens, Phytopathology, 106(6): 591-601. https://doi.org/10.1094/PHYTO-09-15-0214-R Bongomin F., Gago S., Oladele R., and Denning D., 2017, Global and multi-national prevalence of fungal diseases—estimate precision, Journal of Fungi, 3(4): 57. https://doi.org/10.3390/jof3040057 Byrne M., Thapa G., Doohan F., and Burke J., 2022, Lactic acid bacteria as potential biocontrol agents for fusarium head blight disease of spring barley, Frontiers in Microbiology, 13: 912632. https://doi.org/10.3389/fmicb.2022.912632

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