Molecular Microbiology Research 2024, Vol.14, No.3, 119-123 http://microbescipublisher.com/index.php/mmr 122 Figure 4 illustrates the distribution of nrfA sequence fragments from metagenomes across different biomes through a phylogenetic tree. Part A shows the distribution in aquatic environments, while Part B displays terrestrial biomes. Bacterial phyla are color-coded in the outer ring based on genomic classification databases. In the diagram, the size of the dots represents the proportion of sequences in the corresponding biome, providing a visual reference for quantitative analysis. This combination of categorization and quantification lays the foundation for studying the ecology and evolution of NrfA-dependent ammonifying microorganisms in various biomes. The scale represents the rate of amino acid substitution, a common metric for comparing gene sequence differences. Outgroup sequences distinctly different from other biomes are not shown in the diagram. Figure 4 Phylogenetic placement of the metagenomic nrfA sequence fragments across biomes 2 Analysis of Research Findings This study provides a comprehensive insight into how NrfA-dependent ammonifying microorganisms facilitate the nitrogen cycle. These microorganisms not only help retain nitrogen within ecosystems but also contribute to reducing greenhouse gas emissions by minimizing nitrate loss. 3 Evaluation of the Research This study is methodologically rigorous and addresses significant gaps in our understanding of nitrogen-cycling microorganisms. However, the research would benefit from broader geographic sampling to enhance the generalizability of the findings.
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