Molecular Microbiology Research 2024, Vol.14, No.3, 119-123 http://microbescipublisher.com/index.php/mmr 120 Figure 1 Schematic illustration of the inorganic nitrogen cycle Figure 2 Structure of NrfA and schematics of the enzyme complexes involved in the electron transport chain from formate to nitrite Figure 3 shows the maximum likelihood phylogenetic tree inferred from the alignment of 1 150 complete NrfA sequences within 1 121 genomic assemblies, based on 350 amino acid positions. Different bacterial phyla are identified by colored bands in the outer ring, with the color coding based on genomic classification databases. Phyla represented by fewer than 10 sequences are labeled as "Others" and shown in gray. Gray branches represent those sequences that contain a Cys-X-X-Cys-His variant at the first heme-binding site. Blue stars mark known bacterial isolates that can reduce nitrate or nitrite to ammonia, while yellow stars indicate bacterial isolates with a determined NrfA crystal structure. The scale bar represents the rate of amino acid substitution (WAG+R10). Outgroups are not shown. This phylogenetic tree reveals the rich diversity of NrfA sequences and their distribution across different bacterial phyla.
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