Molecular Microbiology Research 2024, Vol.14, No.3, 141-152 http://microbescipublisher.com/index.php/mmr 147 5.3 Case Study: Successful Synergy in Pest Control 5.3.1 Case study 1: Aspergillus oryzae in locust control You et al (2023) study highlighting the successful synergy of Aspergillus oryzae in pest control involves its application against locusts. The study evaluated the virulence of A. oryzae strain XJ-1 on adult locusts (Locusta migratoria) in both laboratory and field trials. The lethal concentration for adult locusts was determined to be 3.58×10⁵ conidia/mL 15 days post-inoculation in laboratory settings. Field-cage experiments revealed that locust mortality reached approximately 92% 15 days after inoculation with 3×10⁵ conidia/m². Furthermore, a large-scale field trial over 666.6 hectares, involving aerial spraying of a water suspension of A. oryzae at 2×10⁸ conidia/mL, demonstrated a significant reduction in locust population densities by 85.4%-94.9% within 31 days (You et al., 2023). 5.3.2 Case study 2: synergistic effects with Bacillus licheniformis In the study on mango disease control by Albarr á n-de la Luz et al. (2023), A. oryzae was tested in combination with Bacillus licheniformis M2-7 against phytopathogenic fungi affecting mango trees. The combined application of these microbes resulted in a significant reduction of fungal growth, with A. oryzae showing a 45% inhibition rate against itself, 40% against Colletotrichum sp., and 35% against Aspergillus niger. The presence of B. licheniformis altered the mycelial structure of the fungi, leading to fragmentation and reduced growth. This synergistic effect highlights the potential of combining A. oryzae with other beneficial microbes for enhanced pest control (Albarrán-de la Luz et al., 2022). Figure 2 Damage caused to the structures of the fungi in antagonism was observed at 40X (Adapt from García-Conde et al., 2023) Image caption: Aspergillus oryzae without bacterial inoculum (a); A. oryzae in the presence of Bacillus licheniformis M2–7 (b) andA. oryzae in the pre- sence of B. licheniformis LYA12 (c); Colletotrichum sp. without bacterial inoculum (d) in the presence of M2–7 (e), in the presence of LYA12 (f) and A. niger without bacterial inoculum (g) in the presence of M2–7 (h) and LYA12 (i), the arrows indicate hyphae malformation (Adapt from García-Conde et al., 2023)
RkJQdWJsaXNoZXIy MjQ4ODYzNA==