Bt Research 2025, Vol.16, No.6, 269-277 http://microbescipublisher.com/index.php/bt 276 Acknowledgments The authors would like to thank all teachers and colleagues who provided guidance and assistance during this research, and for the peer review's revision suggestions. Conflict of Interest Disclosure The authors confirm that the study was conducted without any commercial or financial relationships and could be interpreted as a potential conflict of interest. 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