Bt Research 2025, Vol.16, No.2, 79-85 http://microbescipublisher.com/index.php/bt 84 Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Apirajkamol N., James B., Hogarty T., Mainali B., Taylor P., Roberts J., Rane R., Tay W., and Walsh T., 2025, Mining novel Bacillus thuringiensis toxins and secondary metabolites for invasive fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) control, Austral Entomology, 64(1): e70000. https://doi.org/10.1111/aen.70000 Athieniti E., and Spyrou G., 2022, A guide to multi-omics data collection and integration for translational medicine, Computational and Structural Biotechnology Journal, 21: 134-149. https://doi.org/10.1016/j.csbj.2022.11.050 Ayon N., 2023, High-throughput screening of natural product and synthetic molecule libraries for antibacterial drug discovery, Metabolites, 13(5): 625. https://doi.org/10.3390/metabo13050625 Badran A., Guzov V., Huai Q., Kemp M., Vishwanath P., Kain W., Nance A., Evdokimov A., Moshiri F., Turner K., Wang P., Malvar T., and Liu D., 2016, Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance, Nature, 533: 58-63. https://doi.org/10.1038/nature17938 Blay V., Tolani B., Ho S., and Arkin M., 2020, High-throughput screening: today’s biochemical and cell-based approaches, Drug Discovery Today, 25(10): 1807-1821. https://doi.org/10.1016/j.drudis.2020.07.024 Canzler S., Schor J., Busch W., Schubert K., Rolle-Kampczyk U., Seitz H., Kamp H., Von Bergen M., Buesen R., and Hackermüller J., 2020, Prospects and challenges of multi-omics data integration in toxicology, Archives of Toxicology, 94: 371-388. https://doi.org/10.1007/s00204-020-02656-y Cheng J., and Li X., 2021, Development and application of activity-based fluorescent probes for high-throughput screening, Current Medicinal Chemistry, 29(10): 1739-1756. https://doi.org/10.2174/0929867328666210525141728 Deans C., Behmer S., Tessnow A., Tamez-Guerra P., Pusztai-Carey M., and Sword G., 2017, Nutrition affects insect susceptibility to Bt toxins, Scientific Reports, 7: 39705. https://doi.org/10.1038/srep39705 Dively G., Kuhar T., Taylor S., Doughty H., Holmstrom K., Gilrein D., Nault B., Ingerson-Mahar J., Whalen J., Reisig D., Frank D., Fleischer S., Owens D., Welty C., Reay-Jones F., Porter P., Smith J., Saguez J., Murray S., Wallingford A., Byker H., Jensen B., Burkness E., Hutchison W., and Hamby K., 2020, Sweet corn sentinel monitoring for Lepidopteran field-evolved resistance to Bt toxins, Journal of Economic Entomology, 114: 307-319. https://doi.org/10.1093/jee/toaa264 Huberman L., 2025, mSphere of influence: high-throughput screens to rapidly assign function to microbial genes, mSphere, 10(2): e00800-24. https://doi.org/10.1128/msphere.00800-24 Jiang W., Ye W., Tan X., and Bao Y., 2025, Network-based multi-omics integrative analysis methods in drug discovery: a systematic review, BioData Mining, 18: 27. https://doi.org/10.1186/s13040-025-00442-z Leavell M., Singh A., and Kaufmann-Malaga B., 2020, High-throughput screening for improved microbial cell factories, perspective and promise, Current Opinion in Biotechnology, 62: 22-28. https://doi.org/10.1016/j.copbio.2019.07.002 Liu Y., Qin Y., Chen T., Lu M., Qian X., Guo X., and Bai Y., 2020, A practical guide to amplicon and metagenomic analysis of microbiome data, Protein and Cell, 12(5): 315-330. https://doi.org/10.1007/s13238-020-00724-8 Lloyd M., 2020, High-throughput screening for the discovery of enzyme inhibitors, Journal of Medicinal Chemistry, 63(19): 10742-10772. https://doi.org/10.1021/acs.jmedchem.0c00523 Mayr L., and Bojanic D., 2009, Novel trends in high-throughput screening, Current Opinion in Pharmacology, 9(5): 580-588. https://doi.org/10.1016/j.coph.2009.08.004 Mendoza-Almanza G., Esparza-Ibarra E., Ayala-Luján J., Mercado-Reyes M., Godina-González S., Hernández-Barrales M., and Olmos-Soto J., 2020, The cytocidal spectrum of Bacillus thuringiensis toxins: from insects to human cancer cells, Toxins, 12(5): 301. https://doi.org/10.3390/toxins12050301 O’Connor E., Micklefield J., and Cai Y., 2024, Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules, Current Opinion in Biotechnology, 87: 103125. https://doi.org/10.1016/j.copbio.2024.103125 Ross S., Yang F., Santiago-González J., Abdelgaffar H., Kerns D., Jurat-Fuentes J., Sun X., Collett D., and Kerns D., 2025, Evaluation of GS-omega/kappa-Hxtx-Hv1a and Bt toxins against Bt-resistant and -susceptible strains of Helicoverpa zea (Boddie) and Spodoptera frugiperda (J.E. Smith), Pest Management Science, 81(7): 3565-3572. https://doi.org/10.1002/ps.8725
RkJQdWJsaXNoZXIy MjQ4ODYzNA==