Bt Research 2025, Vol.16, No.2, 79-85 http://microbescipublisher.com/index.php/bt 83 Figure 2 Advantages and limitations of HTS methods used in microbiome research (Adopted from Liu et al., 2020) Image caption: A Introduction to HTS methods for different levels of analysis. At the molecule-level, microbiome studies are divided into three types: microbe, DNA, and mRNA. The corresponding research techniques include culturome, amplicon, metagenome, metavirome, and metatranscriptome analyses. B The advantages and limitations of various HTS methods for microbiome analysis (Adopted from Liu et al., 2020) 7 Future Directions and Perspectives 7.1 Integration with CRISPR, synthetic biology, and systems biology approaches In the future, high-throughput screening (HTS) will be deeply integrated with cutting-edge technologies such as CRISPR gene editing, synthetic biology, and systems biology. CRISPR can precisely edit the genes of Bt strains or toxins, rapidly establish multi-functional mutant libraries, and provide rich screening targets for HTS. Synthetic biology can assist in the modular design and recombination of Bt toxin genes, creating entirely new insecticidal proteins in terms of structure and function. Systems biology can reveal the action network and regulatory mechanism of toxins by integrating multi-omics data, providing theoretical support for efficient screening and functional optimization (Mayr and Bojanic, 2009). 7.2 Development of rapid field-deployable HTS platforms The future HTS platform will become more portable and flexible with the development of automation and miniaturization technologies, and can be used in the field or in resource-limited environments. The new generation of HTS equipment integrates sample processing, detection and data analysis, and can conduct high-throughput screening of environmental samples, individual pests or soil microorganisms on-site (Mayr and Bojanic, 2009). This can accelerate the discovery of new toxins and real-time monitoring of pest resistance and ecological risks, providing technical support for precision agriculture and green prevention and control. 7.3 Opportunities for public-private collaborations in toxin discovery In the future, the high-throughput screening and application of Bt toxins will rely more on the cooperation among the academic community, enterprises and the government. Public-private partnerships can integrate multiple resources, promote the construction of large-scale libraries, platform development and data sharing, and accelerate the industrialization process of new toxins (Mayr and Bojanic, 2009). This cooperation model is beneficial for establishing standardized screening processes and intellectual property protection mechanisms, as well as promoting the global dissemination and sustainable utilization of Bt toxin innovation achievements. Acknowledgments The authors thank Professor Jian for his continuous guidance throughout the entire research process of this study.
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