Bt Research 2024, Vol.15, No.2, 96-109 http://microbescipublisher.com/index.php/bt 102 across generations, providing a reliable and efficient approach to trait management (Ainley et al., 2013). These findings highlight the advantages of stacked traits over single traits in enhancing crop resilience and performance. 5.3 Lessons learned The successful implementation of gene stacking strategies in Bt crops offers several valuable lessons. The integration of multiple genes can significantly enhance the durability of pest and disease resistance, reducing the likelihood of resistance development in pests. This has been demonstrated in various crops, including rice and maize, where stacked traits have provided more robust and long-lasting resistance compared to single traits (Yang et al., 2011; Ainley et al., 2013). Figure 3 Evaluation of agronomic traits and emergence rates in cotton stacks and single events (Adapted from José et al., 2020) Image caption: A, C, and E show the agronomic traits of different cotton materials (stacked, single, conventional control, and commercial reference materials), including initial plant number, final plant number, plant height, days to 50% flowering, physiological maturity, and seed cotton yield; B, D, and F present the emergence rates of different materials (Adapted from José et al., 2020)
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