International Journal of Molecular Medical Science, 2024, Vol.14, No.4, 239-251 http://medscipublisher.com/index.php/ijmms 249 Acknowledgments The authors extend sincere thanks to two anonymous peer reviewers for their invaluable feedback on the manuscript of this paper. Funding This work was supported by Haiyan Foundation of Harbin Medical University Cancer Hospital (grant No.04000480). Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Ahmed R., Zaman T., Chowdhury F., Mraiche F., Tariq M., Ahmad I., and Hasan A., 2022, Single-cell RNA sequencing with spatial transcriptomics of cancer tissues, International Journal of Molecular Sciences, 23(6): 3042. https://doi.org/10.3390/ijms23063042 PMid:35328458 PMCid:PMC8955933 Azizi E., Carr A., Plitas G., Cornish A., Konopacki C., Prabhakaran S., Nainys J., Wu K., Kiseliovas V., Setty M., Choi K., Fromme R., Dao P., McKenney P., Wasti R., Kadaveru K., Mazutis L., Rudensky A., and Pe’er, D., 2018, Single-cell map of diverse immune phenotypes in the breast tumor microenvironment, Cell, 174: 1293-1308 https://doi.org/10.1016/j.cell.2018.05.060 Bian S., Hou Y., Zhou X., Li X., Yong J., Wang Y., Wang W., Yan J., Hu B., Guo H., Wang J., Gao S., Mao Y., Dong J., Zhu P., Xiu D., Yan L., Wen L., Qiao J., Tang F., and Fu W., 2018, Single-cell multiomics sequencing and analyses of human colorectal cancer, Science, 362: 1060-1063. https://doi.org/10.1126/science.aao3791 Bowes A., Tarabichi M., Pillay N., and Loo P., 2022. Leveraging single‐cell sequencing to unravel intratumour heterogeneity and tumour evolution in human cancers, The Journal of Pathology, 257: 466-478. https://doi.org/10.1002/path.5914 Cha J., and Lee I., 2020, Single-cell network biology for resolving cellular heterogeneity in human diseases, Experimental and Molecular Medicine, 52: 1798-1808. https://doi.org/10.1038/s12276-020-00528-0 Chen D., Luo Y., and Cheng G., 2022, Single cell and immunity: better understanding immune cell heterogeneities with single cell sequencing, Clinical and Translational Medicine, 13(1): e1159. https://doi.org/10.1002/ctm2.1159 Chen J., Zhou Q., Wang Y., and Ning K., 2016, Single-cell SNP analyses and interpretations based on RNA-Seq data for colon cancer research, Scientific Reports, 6(1): 34420. https://doi.org/10.1038/srep34420 Chen S., Zhou Z., Li Y., Du Y., and Chen G., 2023, Application of single-cell sequencing to the research of tumor microenvironment, Frontiers in Immunology, 14: 1285540. https://doi.org/10.3389/fimmu.2023.1285540 PMid:37965341 PMCid:PMC10641410 Chen Y., Wang D., Li Y., Qi L., Si W., Bo Y., Chen X., Ye Z., Fan H., Liu B., Liu C., Zhang L., Zhang X., Li Z., Zhu L., Wu A., Zhang Z., 2024, Spatiotemporal single-cell analysis decodes cellular dynamics underlying different responses to immunotherapy in colorectal cancer, Cancer Cell, 2(7):1268-1285.e7. https://doi.org/doi: 10.1016/j.ccell.2024.06.009 Dalerba P., Kalisky T., Sahoo D., Rajendra P., Rothenberg M., Leyrat A., Sim S., Okamoto J., Johnston D., Qian D., Zabala M., Bueno J., Neff N., Wang J., Shelton A., Visser B., Hisamori S., Shimono Y., Wetering M., Clevers H., Clarke M., and Quake S., 2011, Single-cell dissection of transcriptional heterogeneity in human colon tumors, Nature Biotechnology, 29: 1120-1127. https://doi.org/10.1038/nbt.2038 PMid:22081019 PMCid:PMC3237928 Fan J., Slowikowski K., and Zhang F., 2020, Single-cell transcriptomics in cancer: computational challenges and opportunities, Experimental & Molecular Medicine, 52: 1452-1465. https://doi.org/10.1038/s12276-020-0422-0 Gallois C., Pernot S., Zaanan A., Taieb J., 2018, Colorectal cancer: why does side matter?, Drugs, 78(8): 789-798. https://doi.org/10.1007/s40265-018-0921-7 PMid:29790124 García-Sanz R., and Jiménez C., 2021, Time to move to the single-cell level: applications of single-cell multi-omics to hematological malignancies and Waldenström’s macroglobulinemia-a particularly heterogeneous lymphoma, Cancers, 13(7): 1541. https://doi.org/10.3390/cancers13071541 Gerstung M., Beisel C., Rechsteiner M., Wild P., Schraml P., Moch H., and Beerenwinkel N., 2012, Reliable detection of subclonal single-nucleotide variants in tumour cell populations, Nature Communications, 3(1): 811. https://doi.org/10.1038/ncomms1814
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