International Journal of Molecular Medical Science, 2024, Vol.14, No.2, 48-55 http://medscipublisher.com/index.php/ijmms 50 1.2 Gene therapy related to myocardial protection and reconstruction Gene therapy related to myocardial protection and reconstruction is one of the research hotspots in the field of cardiovascular diseases. By regulating the expression of myocardial protective genes and regeneration related genes, we can promote the survival and regeneration of myocardial cells, providing new pathways for the repair of myocardial tissue and the recovery of cardiac function. However, more research is still needed to achieve the clinical application of gene therapy. Myocardial protection and reconstruction are important directions in the treatment of cardiovascular diseases, and gene therapy also has potential application prospects in this field. Gene therapy research on myocardial protection and reconstruction can promote the survival and regeneration of myocardial cells by activating protective genes, inhibiting harmful genes, etc., thereby reducing myocardial injury and promoting the recovery of cardiac function. A common gene therapy strategy is to transduce protective genes such as heat shock protein (HSP), antioxidant enzymes (such as superoxide dismutase SOD), and anti apoptotic factors (such as Bcl-2). Overexpression of these genes can increase the antioxidant capacity of myocardial cells, reduce cell apoptosis, and promote cell survival and recovery of cardiac function. Another strategy is to promote the regeneration and repair of myocardial cells by transducing regeneration related genes, such as genes that regulate myocardial cell proliferation and differentiation. Some studies have shown that by introducing cell cycle regulatory factors, fibroblast transdifferentiation genes, etc., the proliferation and differentiation of myocardial cells can be stimulated, thereby promoting the reconstruction and repair of myocardial tissue. In addition, gene therapy can also achieve myocardial reconstruction through stem cells and genetically modified cells. For example, by transducing specific genes in stem cells or genetically modified cells, targeted differentiation of these cells into cardiomyocytes can be promoted (Figure 2), thereby achieving myocardial regeneration and reconstruction. Although gene therapy related to myocardial protection and reconstruction has shown great potential in laboratory and animal models, its application in clinical trials still faces some challenges. For example, the delivery system of gene therapy, gene selection, and regulation of expression levels still need further research and optimization. In addition, the evaluation of long-term effectiveness and safety is also an important issue. 1.3 Gene therapy related to inflammation and immune regulation Gene therapy related to inflammation and immune regulation is a new field in the treatment of cardiovascular diseases. By regulating the expression of genes related to inflammation and immune response, we can suppress the degree of inflammation and immune response, thereby improving the prognosis of cardiovascular disease. However, further research is still needed to validate the safety and efficacy of gene therapy in order to realize its potential in clinical applications. Figure 2 Myocardial cells
RkJQdWJsaXNoZXIy MjQ4ODYzNQ==