IJCCR_2024v14n2

International Journal of Clinical Case Reports 2024, Vol.14, No.2, 94-106 http://medscipublisher.com/index.php/ijccr 106 Rodriguez E., 2016, Ethical Issues in Genome Editing using Crispr/Cas9 System, Journal of Clinical Research and Bioethics, 7: 1-4. Rodríguez E., 2017, Ethical Issues in Genome Editing for Non-Human Organisms Using CRISPR/Cas9 System, Journal of Clinical Research and Bioethics, 8: 1-5. https://doi.org/10.4172/2155-9627.1000300 Solhjou Z., Uehara M., Bahmani B., Maarouf O.H., Ichimura T., Brooks C.R., and Abdi R., 2017, Novel Application of Localized Nanodelivery of Anti-Interleukin‐6 Protects Organ Transplant From Ischemia-Reperfusion Injuries, American Journal of Transplantation, 17(9): 2326-2337. https://doi.org/10.1111/ajt.14266 Sun D., Guo Z., Liu Y., and Zhang Y., 2017, Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods, Frontiers in Physiology, 8: 608 https://doi.org/10.3389/fphys.2017.00608 Tanihara F., Hirata M., Nguyen N.T., Sawamoto O., Kikuchi T., and Otoi T., 2021, One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis, International Journal of Molecular Sciences, 22(5): 2249. https://doi.org/10.3390/ijms22052249 Wang R., Ruan M., Zhang R., Chen L., Li X., Fang B., and Dai Y., 2018, Antigenicity of tissues and organs from GGTA1/CMAH/β4GalNT2 triple gene knockout pigs, Journal of Biomedical Research, 33(4): 235-243. https://doi.org/10.7555/JBR.32.20180018 Wang R., Ruan M., Zhang R., Chen L., Li X., Fang B., Li C., Ren X., Liu J., Xiong Q., Zhang L., Jin Y., Li L., Li R., Wang Y., Yang H., and Dai Y., 2018, Antigenicity of tissues and organs from GGTA1/CMAH/β4GalNT2 triple gene knockout pigs, Journal of Biomedical Research, 33(4): 235-243. https://doi.org/10.7555/JBR.32.20180018 Wei T., Cheng Q., Farbiak L., Anderson D.G., Langer R., and Siegwart D., 2020, Delivery of Tissue-Targeted Scalpels: Opportunities and Challenges for In Vivo CRISPR/Cas-Based Genome Editing, ACS Nano, 14(8): 9243-9262. https://doi.org/10.1021/acsnano.0c04707 Wienert B., Wyman S.K., Richardson C.D., Yeh C.D., Akcakaya P., Porritt M.J., and Corn J.E., 2019, Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq, Science, 364(6437): 286-289. https://doi.org/10.1126/science.aav9023 Wolf E., Kemter E., Klymiuk N., and Reichart B., 2019, Genetically modified pigs as donors of cells tissues, and organs for xenotransplantation, Animal Frontiers: The Review Magazine of Animal Agriculture, 9(2): 13-20. https://doi.org/10.1093/af/vfz014 Wu H., Lian M., and Lai L., 2023, Multiple gene modifications of pigs for overcoming obstacles of xenotransplantation, National Science Open, 5(2): 17. https://doi.org/10.1360/nso/20230030 Yoon S., Lee S., Park C., Choi H., Yoo M., Lee S.C., Hyun C.H., Kim N., Kang T., Son E., Ghosh M., Son Y.O., and Hur C., 2022, An Efficacious Transgenic Strategy for Triple Knockout of Xeno-Reactive Antigen Genes GGTA1, CMAH, and B4GALNT2 from Jeju Native Pigs, Vaccines, 10(9): 1503. https://doi.org/10.3390/vaccines10091503 Zhang R., Wang Y., Chen L., Wang R., Li C., Li X., Fang B., Ren X., Ruan M., Liu J., Xiong Q., Zhang L., Jin Y., Zhang M., Liu X., Li L., Chen Q., Pan D., Li R., Cooper D., Yang H., and Dai Y., 2018, Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH, Acta Biomaterialia, 72: 196-205. https://doi.org/10.1016/j.actbio.2018.03.055 Zhang X., Wang Q., Zhao J., Li X., Peng W., Yang Z., Lin Z., Yang L., Ding R., Tao K., and Dou K., 2020, The resurgent landscape of xenotransplantation of pig organs in nonhuman primates, Science China Life Sciences, 64(6): 697-708. https://doi.org/10.1007/s11427-019-1806-2 Zhang X.H., Tee L.Y., Wang X.G., Huang Q.S., and Yang S.H., 2015, Off-target effects in CRISPR/Cas9-mediated genome engineering, Molecular Therapy - Nucleic Acids, 4: e264. https://doi.org/10.1038/mtna.2015.37

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