CGE_2025v13n3

Cancer Genetics and Epigenetics, 2025, Vol.13, No.3, 106-116 http://medscipublisher.com/index.php/cge 114 Acknowledgments We would like to thank Dr. Luo continuous support throughout the development of this study. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Alavizadeh S., Hosseinikhah S., Shahbazi M., Jaafari M., Sahebkar A., Moosavian S., Kesharwani P., Sillanpää M., and Gheybi F., 2022, Role of exosomes in tumour growth, chemoresistance and immunity: state-of-the-art, Journal of Drug Targeting, 31: 32-50. https://doi.org/10.1080/1061186X.2022.2114000 Ayala-Mar S., González‐Valdez J., and Donoso-Quezada J., 2021, The role of lipids in exosome biology and intercellular communication: function, analytics and applications, Traffic (Copenhagen, Denmark), 22: 204-220. https://doi.org/10.1111/tra.12803 Breakefield X., Enderle D., Skog J., Hurley J., Roberts D., Yu W., Noerholm M., and Chakrabortty S., 2021, Exosome-based liquid biopsies in cancer: opportunities and challenges, Annals of Oncology, 32: 466-477. https://doi.org/10.1016/j.annonc.2021.01.074 Burgy O., Bettcher B., Bruce K.D., Coughlan C., Potter H., Chial H., Adame V., Boyd T., Anton P., Garcia-Perez J., Graner M., Michel C., Koenigshoff M., and Hsieh E., 2020, Exosome isolation by ultracentrifugation and precipitation and techniques for downstream analyses, Current Protocols in Cell Biology, 88(1): e110. https://doi.org/10.1002/cpcb.110 Cai H., Yu D., Gu J., Li Y., Xu W., Zhang X., Fang X., and Wang M., 2022, Exosomes as a new frontier of cancer liquid biopsy, Molecular Cancer, 21(1): 56. https://doi.org/10.1186/s12943-022-01509-9 Caruso C., Agnoletto C., Garofalo C., and Pignochino Y., 2023, Exosome-based liquid biopsy approaches in bone and soft tissue sarcomas: review of the literature, prospectives, and hopes for clinical application, International Journal of Molecular Sciences, 24(6): 5159. https://doi.org/10.3390/ijms24065159 Chen C., Tenchov R., Zhou Q., Sasso J., Liaw W., and Wang X., 2022, Exosomes-nature’s lipid nanoparticles, a rising star in drug delivery and diagnostics, ACS Nano, 16: 17802-17846 https://doi.org/10.1021/acsnano.2c08774 Chen T., 2024, Single-cell RNA sequencing reveals new insights into tumor heterogeneity, Cancer Genetics and Epigenetics, 12(1): 55-65. https://doi.org/10.5376/cge.2024.12.0007 Contreras-Naranjo J.C., Wu H.J., and Ugaz V.M., 2017, Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine, Lab on a Chip, 17(21): 3558-3577. Demirci U., Ma P., Wang J., Kim D.H., and Liu B., 2021, Towards microfluidic-based exosome isolation and detection for tumor therapy, Nano Today, 37: 101066. https://doi.org/10.1016/j.nantod.2020.101066 Dong P., Gurunathan S., Li J., Zhang Y., and Yang G., 2023, A comprehensive review on the composition, biogenesis, purification, and multifunctional role of exosome as delivery vehicles for cancer therapy, Biomedicine and Pharmacotherapy, 165: 115087. https://doi.org/10.1016/j.biopha.2023.115087 Du L., Chen J., Zhang T., Li P., Xu Z., Wang R., and Huang X., 2022, Review on strategies and technologies for exosome isolation and purification, Frontiers in Bioengineering and Biotechnology, 9: 811971. https://doi.org/10.3389/fbioe.2021.811971 Edalat P., Jadli A., Ballasy N., and Patel V., 2020, Inside (sight) of tiny communicator: exosome biogenesis, secretion, and uptake, Molecular and Cellular Biochemistry, 467: 77-94. https://doi.org/10.1007/s11010-020-03703-z Feng D., and Krylova S., 2023, The machinery of exosomes: biogenesis, release, and uptake, International Journal of Molecular Sciences, 24(2): 1337. https://doi.org/10.3390/ijms24021337 Fitts C.A., Ji N., Li Y., and Tan C., 2019, Exploiting exosomes in cancer liquid biopsies and drug delivery, Advanced Healthcare Materials, 8(6): 1801268. https://doi.org/10.1002/adhm.201801268 Gao Y., Xie J., Li M., Li R., Yuan H., and Yang Z., 2025, Functional tumor-derived exosomes in NSCLC progression and clinical implications, Frontiers in Pharmacology, 16: 1485661. https://doi.org/10.3389/fphar.2025.1485661 Genova C., Rossi G., Longo L., Coco S., Rijavec E., and Grossi F., 2019, Liquid biopsy in non-small cell lung cancer: highlights and challenges, Cancers, 12(1): 17. https://doi.org/10.3390/cancers12010017 Gopal D., Chakrabarti S., Banik S., Kurian T., and Mazumder N., 2021, Elucidating methods for isolation and quantification of exosomes: a review, Molecular Biotechnology, 63: 249-266. https://doi.org/10.1007/s12033-021-00300-3

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