Medicinal Plant Research 2025, Vol.15, No.6, 264-273 http://hortherbpublisher.com/index.php/mpr 271 analysis, epigenetic profiling, and single-cell or spatial transcriptomics will be required to elucidate cell-specific and developmental regulation mechanisms. Such studies would provide insight into allocation of flux among the distinct phenylpropanoid branches and the creation of characteristic metabolite profiles of A. sinensis. Furthermore, such understanding on phenylpropanoid biosynthesis provides a scientific basis for the innovative use of A. sinensis germplasm and allows for target breeding to improve quality, metabolite profiles, and stress tolerance of this species. Knowledge related to the regulation of biosynthesis will contribute to the development of molecular markers and elite cultivars, as well as standardized cultivation strategies. Moreover, metabolic engineering and synthetic biology enable the sustainable production and at-scale production of high-value bioactives to meet the demands for the creation of new pharmaceuticals and functional foods and health products. In addition, the establishment of integrated biotechnological platforms and enlarged high-throughput screening pipelines will further facilitate the industrialization of phenylpropanoid derivatives and enhance the competitiveness of A. sinensis-based natural products. Acknowledgments The authors extend sincere gratitude to the research team for their meticulous assistance and unwavering support throughout the study's execution and data collection process. We also express heartfelt appreciation to the two anonymous reviewers for their valuable feedback and constructive suggestions during the peer review process, which have significantly contributed to refining the paper's quality and enhancing its academic rigor. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Akinola J., Rajkumar A., and Morrissey J., 2024, Optimisation of coumaric acid production from aromatic amino acids in Kluyveromyces marxianus, Journal of Biotechnology, 11: 110002. https://doi.org/10.1016/j.jbiotec.2024.11.002 Badhan S., Ball A., and Mantri N., 2021, First report of CRISPR/Cas9 mediated DNA-free editing of 4CL and RVE7 genes in chickpea protoplasts, International Journal of Molecular Sciences, 22(1): 396. https://doi.org/10.3390/ijms22010396 Chen L., Fan B., Wang F., Song Y., Wang X., Meng Y., Chen Y., Xia Q., and Sun J., 2024, Research progress in pharmacological effects and mechanisms of Angelica sinensis against cardiovascular and cerebrovascular diseases, Molecules, 29(21): 2100. https://doi.org/10.3390/molecules29092100 Chen L., Li L., Wang F., Hu S., Ding T., Wang Y., Huang Y., Fan B., and Sun J., 2023, Targeted metabolomics study on the effect of vinegar processing on the chemical changes and antioxidant activity of Angelica sinensis, Antioxidants, 12(12): 2053. https://doi.org/10.3390/antiox12122053 Du R., Zhuo Y., Xu J., Ming C., and Chen J., 2022, Transcriptome analysis reveals gene expression changes during repair from mechanical wounding in Aquilaria sinensis, Forests, 13(8): 1258. https://doi.org/10.3390/f13081258 Feng W., Liu P., Yan H., Yu G., Zhang S., Jiang S., Shang E., Qian D., and Duan J., 2022, Investigation of enzymes in the phthalide biosynthetic pathway in Angelica sinensis using integrative metabolite profiles and transcriptome analysis, Frontiers in Plant Science, 13: 928760. https://doi.org/10.3389/fpls.2022.928760 Han X., Li C., Sun S., Ji J., Nie B., Maker G., Ren Y., and Wang L., 2022, The chromosome-level genome of female ginseng (Angelica sinensis) provides insights into molecular mechanisms and evolution of coumarin biosynthesis, The Plant Journal, 112(5): 1224-1237. https://doi.org/10.1111/tpj.16007 Harvey C., Tang M., Schlecht U., Horecka J., Fischer C., Lin H., Li J., Naughton B., Cherry J., Miranda M., Li Y., Chu A., Hennessy J., Vandova G., Inglis D., Aiyar R., Steinmetz L., Davis R., Medema M., Sattely E., Khosla C., St. Onge R., Tang Y., and Hillenmeyer M., 2018, HEx: A heterologous expression platform for the discovery of fungal natural products, Science Advances, 4(3): 5459. https://doi.org/10.1126/sciadv.aar5459 Hou C., Yin M., Lan P., Wang H., Nie H., and Ji X., 2021, Recent progress in the research of Angelica sinensis (Oliv.) Diels polysaccharides: extraction, purification, structure and bioactivities, Chemical and Biological Technologies in Agriculture, 8(1): 14. https://doi.org/10.1186/s40538-021-00214-x Jing M., Wang J., Zhang G., Ou X., Wu N., and Yao K., 2024, Exploring the synergistic effects of soil nutrients, rhizosphere fungi, and endophytic fungi on the shaping of root metabolites in Angelica sinensis (Oliv.) Diels, Fungal Biology, 129(1): 101533. https://doi.org/10.1016/j.funbio.2024.101533
RkJQdWJsaXNoZXIy MjQ4ODYzNA==