Medicinal Plant Research 2025, Vol.15, No.4, 151-160 http://hortherbpublisher.com/index.php/mpr 157 Figure 2 All figures from the micropropagation process. A. Shoot proliferation from stem segments, which were cultured on MS medium supplemented with 1.0 mg/L BA + 0.05 mg/L NAA under cool white light for 60 d. B. Shoot proliferation from terminal buds, which were cultured on MS medium supplemented with 1.0 mg/L BA + 0.05 mg/L NAA under cool white light for 60 d. C. Shoot proliferation from stem segments, which were cultured on 1/4 MS medium for 60 d. D. Shoot proliferation from terminal buds, which were cultured on 1/4 MS medium for 60 d. E. Shoot proliferation from stem segments, which were cultured on B5 medium for 60 d. F. Shoot proliferation from terminal buds, which were cultured on B5 medium for 60 d. G. Shoot proliferation from stem segments, which were cultured under R:B = 1:1 for 60 d. H. Shoot proliferation from terminal buds, which were cultured under R:B = 1:1 for 60 d. I. Shoot proliferation from stem segments, which were cultured under R for 60 d. J. Shoot proliferation from terminal buds, which were cultured under R for 60 d. K. Rooting in MS medium supplemented with 1.0 mg/L IBA + 1.0 mg/L NAA under cool white light for 50 d. L. Well-rooted plants were transplanted into pots with 3:1 peat and vermiculite for 30 d (Adopted from Zhang et al., 2025b) 7 Mechanistic Insights into Root Adaptation 7.1 Integrated mechanisms of root recovery The root adaptation of A. roxburghii, involves the interaction of complex physiological and biochemical processes. Under stress conditions, such as low phosphorus or high temperature, the root system will show changes in the activity of antioxidant enzymes, like SOD, CAT, POD, accumulation of osmotic protective substances (proline, soluble sugar), and regulation of reactive oxygen species (ROS) levels. These reactions are closely linked: enhanced antioxidant activity helps to reduce oxidative damage, while osmotic protective substances stabilize cell structure and maintain metabolic function (Zhang et al., 2024). Plant hormones, especially strigolactone, play a role in coordinating root adaptation. It can promote root elongation, regulate phosphorus absorption, and reduce oxidative stress (Zhong et al., 2025). Transcriptomic analysis revealed that, hormone signaling pathways, antioxidant systems, and metabolic networks, including protein and secondary metabolite synthesis, were synergistically regulated during stress adaptation, thereby ensuring root survival and recovery (Zhang et al., 2024; Zhong et al., 2025).
RkJQdWJsaXNoZXIy MjQ4ODYzNA==