Medicinal Plant Research 2025, Vol.15, No.3, 119-128 http://hortherbpublisher.com/index.php/mpr 127 hormones and environmentally friendly treatments (e.g., strigolactone, polyamines, and customized photolithoplasm), are worthy of promotion in commercial seedling cultivation and the production of medicinal components, which will provide support for the sustainable cultivation and resource utilization of A. roxburghii. Acknowledgments The authors sincerely thank Dr. Li for reviewing the manuscript and providing valuable suggestions, which contributed to its improvement. Additionally, heartfelt gratitude is extended to the two anonymous peer reviewers for their comprehensive evaluation of the manuscript. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Bin Y., Liu S., Xie T., Feng W., Li H., Ye Z., Dong Z., and Qiu Y., 2022, Three new compounds from Anoectochilus roxburghii (Wall.) Lindl., Natural Product Research, 37(19): 3276-3282. https://doi.org/10.1080/14786419.2022.2070746 Cui M., Liang Z., Liu Y., Sun Q., Wu D., Luo L., and Hao Y., 2023, Flavonoid profile of Anoectochilus roxburghii (Wall.) Lindl. under short-term heat stress revealed by integrated metabolome, transcriptome, and biochemical analyses, Plant Physiology and Biochemistry, 201: 107896. https://doi.org/10.1016/j.plaphy.2023.107896 Gam D., Khoi P., Ngoc P., Linh L., Hưng N., Anh P., Thư N., Hien N., Khanh T., and Ha C., 2020, LED lights promote growth and flavonoid accumulation of Anoectochilus roxburghii and are linked to the enhanced expression of several related genes, Plants, 9(10): 1344. https://doi.org/10.3390/plants9101344 Hong R., Wu P., Lin J., Huang L., Yang L., Wu Y., and Yao H., 2020, Three-phase hollow fiber liquid-phase microextraction combined with HPLC for determination of three trace acidic plant growth regulators in Anoectochilus roxburghii (Wall.) Lindl., Journal of Separation Science, 43(14): 2773-2783. https://doi.org/10.1002/jssc.201901321 Huang X., Ouyang K., Luo Y., Xie G., Yang Y., and Zhang J., 2022, A comparative study of characteristics in diploid and tetraploid Anoectochilus roxburghii, Frontiers in Nutrition, 9: 1034751. https://doi.org/10.3389/fnut.2022.1034751 Li H., Cheng C., Chen M., Li Y., Li J., Ye W., and Sun G., 2024, Integrated analysis of miRNA–mRNA regulatory networks in Anoectochilus roxburghii in response to blue laser light, Tree Physiology, 44(12): 144. https://doi.org/10.1093/treephys/tpae144 Liu S.S., Chen J., Li S.C., Zeng X., Meng Z.X., and Guo S.X., 2015, Comparative transcriptome analysis of genes involved in GA–GID1–DELLA regulatory module in symbiotic and asymbiotic seed germination of Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae), International Journal of Molecular Sciences, 16(12): 30190-30203. https://doi.org/10.3390/ijms161226224 Liu Y., Tang T., Duan S., Li C., Lin Q., Wu H., Liu A., Hu B., Wu D., Li S., Shen L., and Wu W., 2020, The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii, Food & Function, 11(4): 3730-3740. https://doi.org/10.1039/c9fo00860h Qiu Y., Song W., Yang Y., Zhou G., Bai Y., Akihisa T., Ye F., Feng F., Zhang W., and Zhang J., 2023, Isolation, structural and bioactivities of polysaccharides from Anoectochilus roxburghii (Wall.) Lindl.: A review, International Journal of Biological Macromolecules, 236: 123883. https://doi.org/10.1016/j.ijbiomac.2023.123883 Shao Q., Wang H., Guo H., Zhou A., Huang Y., Sun Y., and Li M., 2014, Effects of shade treatments on photosynthetic characteristics, chloroplast ultrastructure, and physiology of Anoectochilus roxburghii, PLoS One, 9(2): e85996. https://doi.org/10.1371/journal.pone.0085996 Sun X., Lv A., Chen D., Zhang Z., Wang X., Zhou A., Xu X., Shao Q., and Zheng Y., 2023, Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism, Agricultural Water Management, 289: 108538. https://doi.org/10.1016/j.agwat.2023.108538 Wang H., Chen X., Yan X., Xu Z., Shao Q., Wu X., Tou L., Fang L., Wei M., and Wang H., 2022, Induction, proliferation, regeneration and kinsenoside and flavonoid content analysis of the Anoectochilus roxburghii (Wall.) Lindl. protocorm-like body, Plants, 11(19): 2465. https://doi.org/10.3390/plants11192465 Wang L., Chen Q., Jiang T., and Tang C., 2020, Effect of Anoectochilus roxburghii flavonoids extract on H₂O₂-induced oxidative stress in LO2 cells and D-gal induced aging mice model, Journal of Ethnopharmacology, 254: 112670. https://doi.org/10.1016/j.jep.2020.112670 Wang W., Su M., Li H., Zeng B., Chang Q., and Lai Z., 2018, Effects of supplemental lighting with different light qualities on growth and secondary metabolite content of Anoectochilus roxburghii, PeerJ, 6: e5274. https://doi.org/10.7717/peerj.5274
RkJQdWJsaXNoZXIy MjQ4ODYzNA==