Medicinal Plant Research 2025, Vol.15, No.2, 80-87 http://hortherbpublisher.com/index.php/mpr 81 With reference to the research on the interaction between matrix properties and plant physiological response, the present work will identify key matrix properties that promote seedling performance (Chen et al., 2019; Lin et al., 2021). In addition, the research will develop scientifically based rules to propose appropriate transplanting substrate choice and optimization plans for research and industrial manufacture. The conclusions of this research will provide an excellent reference for clematis culture technology, promote the utilization of high-efficiency transplanting technology, and drive this medicine crop toward sustainable industrialization (Zhang et al., 2017; Zhou et al., 2022). By raising key questions during Melematis' early developmental stage, this work also has broader applications for tissue culture-based medicinal plant resource preservation and reproductive technology innovation. 2 Research Status of Anoectochilus roxburghii Tissue Culture Seedlings and Transplanting Substrates 2.1 Major bioactive compounds and medicinal value of Anoectochilus roxburghii Anoectochilus roxburghii, a perennial herb of the family Orchidaceae, is famous due to its enormous medicinal virtues. The herb comprises a number of bioactive molecules responsible for its curative effects, such as anti-inflammatory, antioxidant, and immunomodulatory properties. These effects render it an exemplary plant in traditional medicine and contemporary pharmacology (Yao et al., 2024). 2.2 Physical and chemical properties of substrates and their effects on plant growth Substrate should be selected to ensure proper growth and development of Anoectochilus roxburghii tissue culture seedlings. Some of the commonly applied substrates are fine sand, peat soil, and organic substrates such as peanut shells and bark. They provide nutrients, enhance water holding capacity, and provide improved air permeability, closely mimicking the plant growing conditions in the natural habitat. These conditions allow for root establishment and general health of the plant, thereby increasing the percentage survival of the transplanted seedlings (Ye et al., 2017). 2.3 Progress in domestic and international research on substrate selection for Anoectochilus roxburghii Anoectochilus roxburghii substrate selection studies have been going on successfully, with researchers experimenting with various combinations to find the optimal conditions for growth and development. For instance, it has been discovered that a combination of decomposing soil and vermiculite is effective since it results in a high rate of survival when grown under shade conditions. In addition, the use of banana in culture media was discovered to enhance seedling growth, including leaf numbers, root growth, and fresh weight (Gam et al., 2020). All these findings hint at ongoing growth of substrate composition optimization for improved cultivation outcomes. 2.4 Technical challenges and development directions in transplanting substrate research Even though a lot has been done, there are also some technical challenges in the research on substrate transplanting of Anoectochilus roxburghii. One of them is the maintenance of the quality and homogeneity of the substrates, which can shift depending on raw material sources and preparation methods. Extensive research is also needed to ascertain the long-term effects of substrates on yield and plant health. Future research needs to work towards developing standard substrate formulations and explore new materials to be utilized for further enhancing tissue culture seedlings growth and survival (Li et al., 2017). 3 Effects of Transplanting Substrates on the Survival Rate of Tissue Culture Seedlings 3.1 Mechanisms of substrate physical and chemical properties affecting survival rate Physical and chemical properties of transplanting media are crucial in determining seedling survival rate of Anoectochilus roxburghii tissue culture seedlings. Peat, river sand, and bark have proved to contain ideal conditions for seedling survival since they hold water and provide good aeration. The combination of river sand and peat, for instance, offers a balanced substrate in which both water retention and drainage are encouraged, which is important in avoiding root rot and guaranteeing healthy growth. The chemical composition of the
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