Medicinal Plant Research 2025, Vol.15, No.2, 62-70 http://hortherbpublisher.com/index.php/mpr 66 killed by heat without extreme tissue damage in the plant. The treated tissue is then transferred into a sterile tissue culture medium where virus-free seedling multiplication and differentiation can occur (Pasternak and Steinmacher, 2024). Through the use of both methods' advantage, the process guarantees healthy pathogen destruction and healthy regeneration (Linck et al., 2019). 5.2 Advantages of combined technology Double heat treatment combined with tissue culture enhances significantly the effectiveness of detoxification in Lindera aggregata. Double treatment provides higher detoxification percentages than separate treatments by affecting more than a single viral activity stage. Heat treatment suppresses active viral replication, and tissue culture grows plants from virus-free meristematic tissues. The process also allows for the mass production of seedlings with retained resistance characteristics and vigor and is therefore scalable for commercial purposes (Torres et al., 2000). 5.3 Optimization of technical parameters Control of the conditions of combined heat treatment and tissue culture is crucial to allow the maximum efficiency and least damage to the tissue in Lindera aggregata. Synergy of heat treatment temperature and time is also required; for example, heating at 37 °C-40 °C for 2-3 weeks has been effective in breaking down viral activity without lowering tissue viability. Subsequently, precise regulation of culture conditions, such as media composition and light patterns, subsequently enhances regeneration success. The process also readily supports diverse virus strains, and hence is a useful tool in plant detoxification programs (Linck et al., 2019). 6 Effectiveness Evaluation of Combined Detoxification Technology 6.1 Detoxification rate and health assessment The effectiveness of virus elimination in Lindera aggregata through the combined application of heat treatment and tissue culture techniques is primarily evaluated by detecting viral loads before and after treatment. Techniques such as reverse transcription-polymerase chain reaction (RT-PCR) are widely used for precise quantification of viral content. Studies have shown that after the application of combined virus elimination methods, viral loads are significantly reduced, and the health status of plant tissues is improved. In addition, growth parameters such as plant height, leaf area, and root length are commonly measured to comprehensively assess the health level of virus-free seedlings (Linck et al., 2019). 6.2 Seedling quality and genetic stability Compound detoxification technology not only improves the quality of Lindera aggregata seedlings but also ensures genetic stability. Experiments on medicinal components such as detoxified seedlings' essential oils and polysaccharides have revealed equivalent active ingredient levels, which confirm that detoxification does not have any negative impact on their pharmacological activity. Genetic stability analysis, which is typically done under the direction of molecular markers like SSR and SNP, ensures that tissue culture does not generate any significant genetic variation in the seedlings produced (Torres et al., 2000). 6.3 Field performance and adaptability Field trials are of utmost importance in assessing the performance and viability of detoxified seedlings. Healthy seedlings produced using the combined detoxification technology display improved growth performance and tolerance to environmental stresses. This work shows lowered disease incidence rates and improved yields in the detoxified plants compared to the untreated controls. These findings demonstrate the potential for this integrated technology in enhancing the vigor and productivity of Lindera aggregata under different conditions of cultivation. 7 Promotion and Industrial Application of the Technology 7.1 Large-scale propagation of healthy seedlings The heat treatment and tissue culture technology has established a repeatable protocol for propagation of Lindera aggregata healthy seedlings. The protocol follows sterilization protocol, optimized heat treatment time and temperature for every step, and plant growth regulators in the culture media preparation. Additionally, callus culture and suspension cell culture provide platforms for controlled synthesis of secondary metabolites, and gene
RkJQdWJsaXNoZXIy MjQ4ODYzNA==