Medicinal Plant Research 2025, Vol.15, No.2, 62-70 http://hortherbpublisher.com/index.php/mpr 64 a period of time that inhibits or inactivates heat-sensitive viral nucleic acids and proteins. In the process, the content of viruses in infected tissues is reduced without utilizing chemical agents, making it an eco-friendly method (Gan et al., 2009). Tissue culture facilitates heat treatment with the cultivation of virus-free plants on sterile, controlled conditions. Meristematic tissues, in which there is minimal possibility of virus infection, are excised and cultured on nutrient media to generate healthy seedlings. The synthesis leads to virus elimination as well as pathogen-free plant production at a higher rate. The combined approach has been found to have high detoxification efficiency, increased seedling vigor, and increased scalability for commercial propagation (Huang et al., 2023). Figure 1 Proportion of chemical compounds inL. aggregata(Adopted from Lv et al., 2023) 3 Application of Heat Treatment inLindera aggregata Detoxification 3.1 Principles of heat treatment Heat treatment is a well-documented virus suppression and elimination method in plants. The primary mechanism is inoculating plant tissue with high temperature, disrupting viral proteins and nucleic acid and hindering replication. Experiments have confirmed that in Lindera aggregata detoxification, viruses exhibit differential temperature and treatment time sensitivity. For instance, thermotherapy at 37 °C-40 °C has been effective in reducing the activity of well-known plant viruses like Tobacco mosaic virus and Potato virus Y (Wang et al., 2018). 3.2 Optimization of heat treatment conditions Optimizing the conditions of heat treatment is the key to achieving high detoxification efficiency and less tissue damage in Lindera aggregata. Temperature gradient tests confirmed that a temperature gradient from 35 °C to
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