Medicinal Plant Research 2025, Vol.15, No.2, 62-70 http://hortherbpublisher.com/index.php/mpr 62 Research Insight Open Access Application of Heat Treatment and Tissue Culture Combined Detoxification Technology in the Healthy Seedling Propagation of Lindera aggregata Xiazhen Huang1 , Yufen Wang 2 1 Tropical Medicinal Plant Research Center, Hainan Institute of Tropical Agricultural Resources, Sanya, 572025, Hainan, China 2 Traditional Chinese Medicine Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China Corresponding author: xiazhen.huang@hitar.org Medicinal Plant Research, 2025, Vol.15, No.2 doi: 10.5376/mpr.2025.15.0007 Received: 02 Jan., 2025 Accepted: 08 Feb., 2025 Published: 15 Mar., 2025 Copyright © 2025 Huang and Wang, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Huang X.Z., and Wang Y.F., 2025, Application of heat treatment and tissue culture combined detoxification technology in the healthy seedling propagation of Lindera aggregata, Medicinal Plant Research, 15(2): 62-70 (doi: 10.5376/mpr.2025.15.0007) Abstract This study discusses the application of integrated heat treatment and tissue culture technology for detoxifying Lindera aggregata seedlings, determining its efficiency in detoxification and healthy seedling development, and its usability and value for further implication. The study has revealed that tissue culture technology readily increases the rate of propagation of Lindera aggregata seedlings using shoot tip culture technology with optimal media conditions promoting shoot proliferation and differentiation. Integrated technology enhances substantially detoxification efficacy along with genetic stability and resistance characteristics of Lindera aggregata seedlings. Thermal treatment suppresses viral replication at elevated temperatures, whereas tissue culture utilizes sterile environment and shoot tip isolation to remove viruses. Combined, these treatments attain detoxification efficacy of over 90%. Detoxified seedlings show better growth characteristics and increased quantities of medicinal constituents, including essential oils and polysaccharides, than control seedlings. The detoxified seedlings also have enhanced growth vigor and stress resistance as well as remarkable active ingredient content promotion. The integration of heat treatment and tissue culture technology in the cultivation of healthy Lindera aggregata seedlings not only increases detoxification efficiency and seedling multiplication but also reduces the risk of disease transmission, rationalizes cultivation management, and guarantees the quality of Lindera aggregata products. This research provides theoretical foundations and technical backstopping for Lindera aggregata germplasm resource preservation and utilization in industry. Keywords Lindera aggregata; Detoxification technology; Heat treatment; Tissue culture; Healthy seedlings 1 Introduction Lindera aggregata, which is a Chinese herbal medicine, is highly medicinally active and finds widespread applications in medicine and healthcare (Salleh, 2020). It is a key component of traditional medicines such as the Suoquan pill that is used in the management of chronic kidney disease (CKD) (Cai et al., 2020). The plant's bioactive phytoconstituents, namely the isoquinoline alkaloids, have extensive applications in the food and drug industries due to their drug potential (Peng et al., 2020). Lindera aggregata has also been used traditionally to treat gastrointestinal diseases since centuries, which exhibits the drug's extensive medicinal applications (Lai et al., 2021). Nevertheless, seedling propagation of Lindera aggregata is highly limited by the incidence of systemic infections, such as viral pathogens, that cannot be eliminated through regular cultivation techniques (Wang et al., 2012). Not only do these infections lower productivity but also hinder industrial manufacturing of quality Lindera aggregata products. Heat treatment and tissue culture are among the new methods that hold the solution to decontamination of infected plants and producing healthy seedlings. Heat treatment suppresses viral activity by exposing plant tissues to elevated temperatures, and tissue culture re-establishes pathogen-free plants from meristematic tissues under aseptic conditions. Combination of the two technologies has been highly promising in detoxifying crops and supporting sustainable seedling propagation (Linck et al., 2019). Detoxification technology when combined with tissue culture methods is a solution for addressing the pathogenic infection challenges of Lindera aggregata cultivation. Tissue culture technology has been successfully implemented in other species such as Lindera glauca, for the aim of increasing propagation levels and ensuring
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