Medicinal Plant Research 2024, Vol.14, No.6, 334-344 http://hortherbpublisher.com/index.php/mpr 342 great result. But what works in one place might not work as well in another. So, testing different mixes or adding other helpful materials could lead to even better growth. Besides the soil, getting the right temperature and humidity also matters a lot. Some researchers have used carefully planned experiments—like orthogonal designs—to find the best lighting setups for boosting growth and compound production (Chen et al., 2021). Using the same approach to fine-tune humidity or temperature might also help, but it’s tricky to get consistent results in different environments. That’s why more detailed studies are needed. Another promising idea is using advanced micropropagation techniques. Some studies show that protocorm-like bodies (PLBs) can be used to grow more plants and even produce useful compounds like kinsenoside (Wang et al., 2022). However, it’s not easy to add this method to regular transplanting steps just yet. It still needs more testing and adjustment. 7.2 Sustainable cultivation and conservation strategies Since A. roxburghii is both valuable and endangered, finding better ways to grow and protect it is very important. One good method is to improve the growing conditions in the lab so the plant can make more useful compounds like polysaccharides and kinsenoside. Jin et al. (2018) showed that certain types of growing media, when combined with the right plant hormones, can help the plant produce more of these helpful substances in rhizome cultures. This means we can rely less on picking plants from the wild. But even with these advances, it’s still hard to fully replace natural sources, because lab results can change from place to place. Another key step is setting up strong and reliable ways to grow lots of plants. Using well-designed growth media and standard growing steps, we can produce large numbers of seedlings in lab conditions (Ru, 2015). This helps reduce the need to collect wild plants, which is good for both conservation and farming. Still, moving the lab-grown plants outdoors is not always easy. If the change isn’t handled carefully, many seedlings might not survive. 7.3 Integrating AI and precision agriculture in transplantation optimization Using artificial intelligence (AI) along with precision farming tools could help improve how A. roxburghii seedlings are transplanted. AI can look at a lot of growing data and find patterns that show the best conditions for healthy growth (Castillo, 2023; Kim and AlZubi, 2024). This helps farmers and researchers adjust their methods in real time to better match the plants’ needs. Still, even the best AI predictions need to be tested in real-life situations, since plants don’t always grow exactly as expected. Precision agriculture uses tools like sensors and automated systems to control things such as temperature, humidity, and light. For example, light sensors can notice when the light is too weak or too strong and adjust it automatically. This helps reduce plant stress and supports better photosynthesis (Chen et al., 2021). But these systems aren’t perfect—sometimes they break down or need to be recalibrated. Even with a few challenges, combining AI with smart farming tools offers a powerful way to improve transplant success. It can make growing A. roxburghii more efficient and also support its long-term protection. Acknowledgments The authors thank the two anonymous reviewers for their feedback on the manuscript of this study. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. Reference Al-Alwani A.A.M., and Mohammed M.A., 2023, Propagation of chia plants using plant tissue culture technique, Journal of Biotechnology Research Center, 17(1): 66-80. https://doi.org/10.24126/jobrc.2023.17.1.702
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