Medicinal Plant Research 2024, Vol.14, No.6, 334-344 http://hortherbpublisher.com/index.php/mpr 341 Figure 3 Induction, proliferation, and regeneration of A. roxburghii PLB. (a) Induction of A. roxburghii PLBs. The arrows indicate stem nodes near apical shoot. (b) Magnification (4×) of the selected area in panel a. (c) Magnification (4×) of the selected area in panel d. (d) Secondary PLB induction. (e) Mastoid PLB mass. (f) Shoot formation. (g) Root formation (the roots are within the circled region) (Adopted from Wang et al., 2022) Image caption: The results showed that under optimized culture conditions (MS + 3 mg/L 6-BA + 0.5 mg/L NAA + 0.8 mg/L ZT + 0.2 mg/L 2,4-D), the PLB induction rate reached 89%, and the secondary PLB induction rate reached 120%. Dark or low light conditions facilitated the proliferation of PLBs, while light promoted their differentiation to intact plants.The high proliferation rate of PLBs provides an effective strategy for the rapid propagation of A. roxburghii, and also supports the potential of PLBs as a platform for secondary metabolite production (Adapted from Wang et al., 2022) Another successful protocol focused on the large-scale in vitro propagation of A. roxburghii, which is crucial for both conservation and commercial purposes. This method utilized nodal segments from field-grown plants as explants and optimized the shoot generation and proliferation processes. The study identified half-strength Murashige and Skoog (MS) medium supplemented with 1.5 mg/L 6-benzyladenine (BA) as optimal for shoot induction, achieving a formation rate of 91.67% (Zhang et al., 2015). For rooting, a medium containing 0.6 mg/L NAA, 0.3 mg/L indole-3-butyric acid (IBA), and 100 mg/L banana mashes was used, resulting in a root induction rate of 93.33%. The rooted plantlets were then successfully acclimatized in a mixture of sterile sand and peat soil, achieving a survival rate of 90.2%. 7 Future Perspectives and Challenges 7.1 Potential improvements in transplantation protocols Even though transplanting A. roxburghii seedlings has gotten better over time, there’s still a lot of room to improve the process. One way to do this is by fine-tuning the soil mix. For example, Zhu (2015) found a mix of peat, bark, and other materials in a 14:1:5 ratio led to a high survival rate—up to 98.1% after 90 days. That’s a
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