Medicinal Plant Research 2024, Vol.14, No.6, 334-344 http://hortherbpublisher.com/index.php/mpr 339 pigments (Gam et al., 2021). But the amount of light matters, too. A strong mix of red and blue light can help plants grow faster. Still, if the light is too bright, it can actually stress the seedlings instead of helping them (Chen et al., 2021). Chlorophyll—the green pigment that helps plants use light—also goes up under red and blue LED lighting. More chlorophyll means the plant can take in more light and turn it into energy (Gam et al., 2020). However, plants don’t adjust right away. When the lighting changes suddenly, photosynthesis may drop at first before it gets better. That’s why it’s important to give the seedlings a balanced light setup. It helps them slowly get used to the new conditions and grow more successfully after transplanting. 5.2 Antioxidant enzyme activities and stress tolerance mechanisms Antioxidant enzymes play an important role in alleviating environmental stress during the transplantation of A. roxburghii seedlings. These enzymes can effectively help plants resist cell damage caused by adversity, thereby enhancing overall stress resistance. At the same time, changes in the external environment can also affect the synthesis of beneficial compounds with antioxidant activity in plants, such as flavonols in polysaccharides and flavonoids, which have strong ability to scavenge free radicals and play a positive role in the plant's antioxidant defense system. Jin et al. (2018) pointed out that adding yeast extract to the culture medium can enhance the antioxidant capacity of A. roxburghii seedlings, help them remove harmful substances such as free radicals, and reduce cell damage. However, the effectiveness of this effect is greatly affected by variety differences and specific cultivation conditions, and the application plan needs to be flexibly adjusted according to the actual situation during the application process. Optimizing the cultivation system is also one of the important strategies to enhance plant stress resistance. For example, adding appropriate combinations of plant hormones to improved MS medium can significantly promote root and shoot development, helping seedlings to smoothly transition from the laboratory to the natural environment (Ru, 2015). Although various measures have been taken to enhance the stress resistance of A. roxburghii, sudden climate change or disease invasion during the transplanting process is still difficult to completely avoid. Therefore, continuous optimization of management methods and cultivation strategies remains an important guarantee for ensuring successful transplantation and healthy growth of seedlings. 5.3 Root system development and water uptake efficiency Having a strong root system is key for A. roxburghii seedlings to take in water and survive after being moved out of the lab. One way to improve root growth is by adjusting the growth medium. Adding the right amount of plant hormones like naphthalene acetic acid (NAA) and 6-benzylaminopurine (6-BA) can help roots grow better (Ru, 2015). When the roots are strong, the plants absorb more water, which helps them grow and survive during acclimatization. However, these hormones must be used carefully. If the amounts are too high, they can actually slow down growth instead of helping. The type of soil or substrate also makes a big difference. Studies show that mixes with peat and bark create a good environment for roots. This kind of substrate helps the plants grow stronger and survive better after transplanting (Zhu, 2015). Still, one mix doesn’t work for every situation. You might need to change it slightly depending on the local climate or soil conditions. 6 Case Studies 6.1 Study on the effects of Ceratobasidiumsp. AR2 on the growth of A. roxburghii tissue culture seedlings Suitable environmental conditions for transplanting, including soil substrate, moisture, light, and utilization of symbiotic microorganisms, are essential for improving the survival of A. roxburghii tissue culture seedlings. A study verified the promotional effect of Ceratobasidium sp. AR2 on the growth and transplantation acclimatization of A. roxburghii tissue culture seedlings through a co-culture experiment (Zhang et al., 2020). In the treatment group inoculated with AR2, A. roxburghii plants exhibited significantly faster growth rate as
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