Medicinal Plant Research 2024, Vol.14, No.6, 320-333 http://hortherbpublisher.com/index.php/mpr 327 Figure 2 Production of spores during the strain cultivation. (A): Spore collection; (B): LZ-1; (C): mutagenic strain UV119 (Adopted from Tang et al., 2023) Image caption: The figure shows the spore production of the Ganoderma lucidumUV119 mutant strain and "Longzhi No.1" over two growth cycles. A shows the spore powder collection process of the UV119 mutant strain, while B and C compare the production of the "Longzhi No.1" and UV119 strains, respectively. The experimental results indicate that UV119's spore yield in the first growth cycle was 26.67% higher than that of "Longzhi No.1," and in the second growth cycle, its spore yield was nearly 10 times that of "Longzhi No.1." This figure reveals the high-yield capacity of the UV119 strain across multiple growth cycles, confirming its significant spore yield advantage over "Longzhi No.1" (Adapted from Tang et al., 2023) 6.2 Optimization of large-scale liquid fermentation process for triterpenoid and sterol production fromG. lucidum G. lucidum, a traditional medicinal fungus in China, is rich in triterpenoids and sterols, which have various bioactive properties such as antitumor, hepatoprotective, and antihypertensive effects (Zhao et al., 2019). However, traditional cultivation methods have low yields and cannot meet the demands of large-scale production. Liquid submerged fermentation, with its shorter production cycle, high controllability, and large output, has become an ideal method for large-scale production of G. lucidum. Feng et al. (2021) conducted an optimization study on large-scale production using liquid submerged fermentation of the G. lucidum G0017 strain. By adjusting different aeration rates, they significantly increased the yield of triterpenoids and sterols. The experiment showed that an aeration rate of 1.25 vvm during the early fermentation stage effectively promoted mycelial growth, while adjusting to 1.5 vvm in the later stage greatly enhanced triterpenoid and sterol synthesis. The optimized process achieved triterpenoid and sterol yields of 3.34 g/L and 3.46 g/L in 3-L and 50-L fermenters, respectively, which were 69.54% and 75.63% higher than the yields under a fixed aeration strategy. This provides important technical support and an optimized plan for large-scale industrial production.
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