Medicinal Plant Research 2024, Vol.14, No.6, 308-319 http://hortherbpublisher.com/index.php/mpr 315 This study addressed critical technical bottlenecks in peony plant regeneration, including low induction rates, browning, and low regeneration efficiency, laying a foundation for large-scale production and genetic improvement of peony. Nevertheless, further optimization of adventitious bud differentiation and rooting rates is required, providing direction for future research. Overall, this system shows significant potential for germplasm preservation, rapid propagation, and genetic engineering applications of Paeonia lactiflora. Figure 3 P. lactiflora shoots rooted in 1/2 MS + 3 g/L AC medium with different auxins: (A) 1.0 mg·L−1 IBA, (B) 0.5 mg·L−1 IAA + 0.5 mg·L−1 IBA. The images shown are of plantlets after 45 days in rooting medium. IAA: indole-3-acetic acid; IBA: indole-3-butyric acid (Adopted from Song et al., 2023) Image caption: The figure illustrates the effects of different auxin concentrations (IBA, IAA) on the rooting of adventitious buds in Paeonia lactiflora. The results show that treatment with 1.0 mg/L IBA resulted in the highest rooting rate (38.89%), with robust root growth, higher root numbers, and a broader coverage area. In contrast, the combined treatment of 0.5 mg/L IAA and 0.5 mg/L IBA showed poor results, with shorter roots and a lower rooting rate (7.78%). The study confirms the significant promotive effect of IBA on rooting in P. lactiflora, outperforming IAA or the combined treatment. These findings provide valuable insights for optimizing rooting conditions inP. lactiflora, particularly in the selection and concentration of auxins (Adapted from Song et al., 2023) 6.2 Optimization strategies for forcing cultivation of ‘Hang Baishao’ The cultivation study of ‘Hang Baishao’ aims to enhance its yield and adaptability, particularly in low-latitude regions. In such areas, warm winter climates hinder bud dormancy release and normal flowering. To overcome this challenge, Zhang et al. (2019a) investigated multiple forcing cultivation strategies, including low-temperature treatment and the combined application of 5-azacytidine and GA3, to optimize flowering time and quality, thereby improving its commercial value. The study revealed that ‘Hang Baishao’ exhibits strong adaptability to warm winter climates in regions like Zhejiang, with a high pollen viability of 55.6%, making it an excellent male parent for hybridization. However, despite good growth, hybrid offspring commonly exhibited flower bud abortion, indicating the need for further optimization of breeding strategies. Moreover, combining chilling treatments with 300 mg/L humic acid irrigation effectively advanced flowering and improved flower quality. Plants treated with artificial chilling and humic acid demonstrated the best performance, achieving early flowering in mid-February and exhibiting excellent ornamental and commercial value (Figure 4). This study systematically addressed the cultivation challenges of peonies in warm winter regions and proposed feasible solutions. On one hand, introducing varieties and hybrid breeding can produce new cultivars with low chilling requirements. On the other hand, employing chilling and exogenous hormone-assisted forcing cultivation techniques enables year-round production of cut flowers and potted peonies. This integrated indoor and outdoor strategy offers valuable insights for the application of other economically significant plants with winter dormancy traits in low-latitude regions.
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