Medicinal Plant Research 2024, Vol.14, No.5, 275-284 http://hortherbpublisher.com/index.php/mpr 280 Figure 2 Summary of BIAs pathway and screening of candidate genes of Corydalis yanhusuo bulb. Heatmap of multiple transcripts by the FPKM values matching each gene is shown side by side near the gene names. The red number in the bracket represents the number of this functional enzyme. Norreticuline 7-O-methyltransferase (N7OMT), berberine bridge enzyme (BBE), scoulerine 9-O-methyltransferase (SOMT), canadine synthase (CAS), stylopine synthase (SPS), (S)-tetrahydroxyprotoberberine oxidase (STOX), columbamine O-methyltransferase (CoOMT), cheilanthifoline synthase (CFS), tetrahydroprotoberberine-N-methyltransferase (TNMT), methylstylopine hydroxylase (MSH), dihydrobenzophenanthridine oxidase (DBOX), protopine-6-hydroxylase (P6H) (Adopted from Zhao et al., 2024) 5.3 Exogenous substance induction and regulation techniques The application of exogenous substances to induce and regulate alkaloid biosynthesis is another effective strategy. For example, the use of specific solvents and pH conditions during the extraction and purification process has been optimized to maximize the yield of various alkaloids in C. yanhusuo (Zhang et al., 2020b). Additionally, environmental factors such as soil physicochemical properties and rhizobacteriome composition, influenced by practices like peanut rotation and flooding, have been shown to significantly impact the growth and medicinal yield of C. yanhusuo (Li et al., 2022). By manipulating these external conditions, it is possible to create an optimal environment that promotes higher alkaloid content in the plant. 6 Case Study 6.1 Study on the differential impact of crop rotation and flooding on yield of C.yanhusuo Traditional cultivation methods often involve planting C. yanhusuo following crop rotation with other plants to enhance land use efficiency. However, the effects of crop rotation can be complex, potentially causing changes in plant growth and medicinal yield. Li et al. (2022) found that compared to fields rotated with peanuts, the growth and medicinal yield of C. yanhusuo were significantly improved in flooded fields (Figure 3). In flooded fields, the enrichment of Acidobacteria was found to help improve plant growth performance, whereas the enrichment of Actinobacteria in rotated fields may have inhibitory effects on the growth of C. yanhusuo. Figure 3 The effect of different field types upon the Corydalis yanhusuo tuber yield. (A) Photographs of C. yanhusuo plants from the three different fields. (B) The total tuber yield per hectare of C. yanhusuo tuber from each field type. PL, plants from the PL field (field under peanut-C. yanhusuo rotation); N, plants from the N field (C. yanhusuo field without rotation and flooding); HR, plants from the HR field (C. yanhusuofield that had been flooded in July, 2020) (Adopted from Li et al., 2022)
RkJQdWJsaXNoZXIy MjQ4ODYzNA==