Medicinal Plant Research 2024, Vol.14, No.2, 85-96 http://hortherbpublisher.com/index.php/mpr 90 particularly lavender essential oil, can significantly reduce preoperative anxiety. Short-term inhalation of aromatherapy has been found to be especially effective and is recommended for clinical application to help alleviate anxiety symptoms (Eslami et al., 2018; Guo et al., 2020). Figure 2 1,2,4-TTB inhibits activation of the NLRP3 inflammasome in vivo (Adopted from Pan et al., 2021) Image caption: (a) Spinal cord slices from EAE model mice administered 1,2,4-TTB or vehicle were stained with an anti-ASC antibody to analyze the expression of ASC. (b-d) The expression of cleaved Casp-1, NLRP3, pro-IL-1β, pro-Casp-1, ASC, and β-actin was analyzed by Western blotting (b), and the gray values of the ASC (c) and cleaved Casp-1 (d) bands were analyzed by ImageJ. (e-h) RNA was extracted from the spinal cords of EAE model mice administered 1,2,4-TTB or vehicle, and the mRNA levels of IFN-γ (e), IL-17a (f), CCL-5 (g), and IL-4 (h) were analyzed by real-time PCR (* indicates P< 0.05, ** indicates P< 0.01 by one-way ANOVA) (Adapted from Pan et al., 2021)
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