Journal of Tea Science Research, 2024, Vol.14, No.6, 344-352 http://hortherbpublisher.com/index.php/jtsr 346 Figure 1 Identification of the key candidate genes involved in the synthesis of O-methylated catechins in tea plants.a Biosynthetic pathway of main O-methylated catechins (EGCG3″Me and EGCG4″Me). SAM, S-adenosyl-L-methionine. OMT Omethyltransferase, SAH S-adenosyl-L-homocysteine. b Chromosome distribution of ED4 (Euclidean distance (ED) raised to the fourth power) values based on SNPs and InDels and differentially expressed genes (DEGs) in the volcano plot. With the chromosomal location of ‘Huangdan’ genome used as the abscissa and the ED4 value used as the ordinate, the chromosomal scores of RNA-seq SNP and InDel ED4 values were plotted. The conditions for screening DEGs were |log2FoldChange | > 1 and P < 0.05 by unpaired two-tailed test. c Phylogenetic analysis of O-methyl- transferases. Phylogenetic tree of selected OMTs: Eucalyptus globulus CCoAOMT (O81185), Eucalyptus gunnii CCoAOMT (O04854), Nicotiana tabacum CCoAOMT (O24151), Solanum tuberosum CCoAOMT (Q8H9B6), Camellia sinensis CCoAOMT (K9USP2), Populus trichocarpa CCoAOMT (O65922), Vitis vinifera FAOMT (C7AE94), Triticum aestivum FOMT (Q84N28), Oryza sativa subsp. Japonica COMT (Q6ZD89), Chrysosplenium americanum FOMT (Q42653), Arabidopsis thaliana COMT (Q9FK25), Camellia sinensis COMT (E2FYC3), Coffea canephora COMT (Q8LL87) (Adopted from Jin et al., 2023) 3.3 Tissue specificity and developmental regulation of biosynthetic pathways Catechin biosynthesis is highly tissue- and development-specific. The highest catechin concentrations are typically found in young leaves, with lower levels in stems and roots (Wang et al., 2018). Transcriptome analyses
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