IJH_2025v15n5

International Journal of Horticulture, 2025, Vol.15, No.5, 218-233 http://hortherbpublisher.com/index.php/ijh 232 Looking to the future, the breeding of fresh sugarcane will shift from relying mainly on field trait selection to precise breeding of the "trait-gene-molecule" trinity: using multi-omics to analyze trait mechanisms, using genomics to lock in favorable genes, and finally using molecular technology to efficiently introduce these genes into new strains. It can be foreseen that in the near future, a batch of new fresh sugarcane varieties with both high sugar content and good taste will be born with the support of multi-omics and molecular breeding, further promoting the quality upgrade of the sugarcane industry. Acknowledgments We thank Mr Y. Ding from the Institute of Life Science of Jiyang College of Zhejiang A&F University for his reading and revising suggestion. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Buckeridge M., Grandis A., and Tavares E. Q.P., 2019, Disassembling the glycomic code of sugarcane cell walls to improve second-generation bioethanol production, in Bioethanol Production from Food Crops, pp.17-35. https://doi.org/10.1016/B978-0-12-813766-6.00002-3 Chen P., Bao H., Chen P., and Shen W., 2022, Field effect evaluation of healthy seed canes of chewing cane, Chinese Agricultural Science Bulletin, 38(16): 1-5. Deng Q., Dou Z., Chen J., Wang K., and Shen W., 2019, Drought tolerance evaluation of intergeneric hybrids of BC3F1 lines of Saccharum officinarum × Erianthus arundinaceus, Euphytica, 215: 85. https://doi.org/10.1007/s10681-019-2513-3 Huang D. D., and Li Z., 2024, Advances in molecular breeding techniques for pitaya (Hylocereus), Tree Genetics and Molecular Breeding, 14(5): 239-246. https://doi.org/10.5376/tgmb.2024.14.0023 Kannan B., Jung J.-H., Moxley G. W., Lee S., and Altpeter F., 2018, TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield, Plant Biotechnology Journal, 16(4): 856-866. https://doi.org/10.1111/pbi.12833 Khan Q., Qin Y., Guo D., Yang L., Song X., Xing Y., and Li Y., 2023, A review of the diverse genes and molecules involved in sucrose metabolism and innovative approaches to improve sucrose content in sugarcane, Agronomy, 13(12): 2957. https://doi.org/10.3390/agronomy13122957 Khan Q., Chen J.Y., Zeng X.P., Qin Y.Z., Guo D.J., Mahmood A., Yang L. T., Liang Q., Song X.P., Xing Y., and Li Y.R., 2021, Transcriptomic exploration of a high sucrose mutant in comparison with the low sucrose mother genotype in sugarcane during sugar accumulating stage, GCB Bioenergy, 13(9): 1448-1465. https://doi.org/10.1111/gcbb.12868 Li H., Chen Y., Zhang C., Zhang S., Hu L., Huang C., Wu H., Liu X., Wang H., and Li Y., 2023, Assessing differential gene expression and metabolic regulation underlying sugar and fiber accumulation in sugarcane genotypes, Plants, 12(5): 1041. https://doi.org/10.3390/plants12051041 Liu Q., Hu P., Zheng M., Lin Z., Li Z., and Li T., 2024, Research on crop tissue structure and mechanical properties, Journal of South China Agricultural University, 45(3): 446-456. Lu Y. Q., He Y.X., Wang Z.X., and Tshibunga F.M., 2024, Molecular mechanisms of cambium formation and activity maintenance: a systematic review of the collaborative regulation of tree stem cells in growth, development, and environmental adaptation, Tree Genetics and Molecular Breeding, 14(5): 218-228. https://doi.org/10.5376/tgmb.2024.14.0021 Martins T. D.S., Magalhães Filho J.R., Cruz L., Machado D.F.S.P., Erismann N. M., Gondim-Tomaz R.M.A., Marchiori P.E.R., Silva A.L.B.O., Machado E.C., and Ribeiro R.V., 2024, Physiological and biochemical processes underlying the differential sucrose yield and biomass production in sugarcane varieties, Experimental Agriculture, 60: e13. https://doi.org/10.1017/S0014479724000061 Mehdi F., Cao Z., Zhang S., Gan Y., Cai W., Peng L., Wu Y., Wang W., and Yang B., 2024a, Factors affecting the production of sugarcane yield and sucrose accumulation: suggested potential biological solutions, Frontiers in Plant Science, 15: 1374228. https://doi.org/10.3389/fpls.2024.1374228 Mehdi F., Javed T., Liu X., Aman J., Riaz A., Galani S., Shaheen T., Nawaz M.A., and Peng L., 2024b, Current perspectives on the regulatory mechanisms of sucrose accumulation in sugarcane, Current Plant Biology, 27: 100240. https://doi.org/10.1016/j.cpb.2024.100240

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