International Journal of Horticulture, 2024, Vol.14, No.3, 142-155 http://hortherbpublisher.com/index.php/ijh 153 8.3 Call for continued research and interdisciplinary collaboration Continued research is essential to refine CRISPR/Cas9 techniques and explore their full potential in controlling ACP and HLB. Interdisciplinary collaboration among geneticists, entomologists, plant pathologists, and agricultural economists is crucial to address the multifaceted challenges posed by HLB. By working together, these experts can develop comprehensive strategies that combine genetic, biological, and chemical control methods to manage ACP populations and reduce the impact of HLB on the citrus industry. Furthermore, engaging with policymakers and the public is vital to ensure the acceptance and ethical use of gene-editing technologies in agriculture. In conclusion, the advancements in CRISPR/Cas9 gene editing for ACP control represent a significant step forward in the fight against HLB. By fostering continued research and collaboration, we can develop innovative solutions that protect citrus crops and support the global citrus industry. Acknowledgments The authors would like to thank Dr. X.J. Fang, General Director of the Hainan Institute of Tropical Agricultural Resources, for reading the manuscript and providing valuable suggestions for revision. Additionally, thanks are extended to the two anonymous peer reviewers for their rigorous review and constructive comments on the paper. Funding This research was supported by the National Natural Science Foundation of China (Grant No. 32060658), Guangxi Key R&D Program (Grant No. Guike AB18294015), and Nanning Major Science and Technology Special Project (Grant No.20232080). Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Ahmad S., Wei X., Sheng Z., Hu P., and Tang S., 2020, CRISPR/Cas9 for development of disease resistance in plants: recent progress, limitations and future prospects, Briefings in Functional Genomics, 19(1): 26-39. https://doi.org/10.1093/bfgp/elz041 Ajene I., Khamis F., Ballo S., Pietersen G., Asch B., Seid N., Azerefegne F., Ekesi S., and Mohamed S., 2020, Detection of Asian citrus psyllid (Hemiptera: Psyllidae) in Ethiopia: a new haplotype and its implication to the proliferation of Huanglongbing, Journal of Economic Entomology, 113: 1640-1647. https://doi.org/10.1093/jee/toaa113 Alba-Tercedor J., Hunter W.B., and Alba-Alejandre I., 2021, Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf, Scientific Reports, 11(1): 1358. Ammar E., Hall D., Hosseinzadeh S., and Heck M., 2018, The quest for a non-vector psyllid: Natural variation in acquisition and transmission of the huanglongbing pathogen ‘Candidatus Liberibacter asiaticus’ by Asian citrus psyllid isofemale lines, PLoS ONE, 13. https://doi.org/10.1371/journal.pone.0195804 Bao A., Burritt D., Chen H., Zhou X., Cao D., and Tran L., 2019, The CRISPR/Cas9 system and its applications in crop genome editing, Critical Reviews in Biotechnology, 39: 321-336. https://doi.org/10.1080/07388551.2018.1554621 Beloti V., Alves G., Coletta-Filho H., and Yamamoto P., 2018, The Asian citrus psyllid host Murraya koenigii is immune to citrus Huanglongbing pathogen 'Candidatus Liberibacter asiaticus', Phytopathology, 108(9): 1089-1094. https://doi.org/10.1094/PHYTO-01-18-0012-R Carlson C., Horst A., Johnston J., Henry E., Falk B., and Kuo Y., 2022, High-quality, chromosome-scale genome assemblies: comparisons of three Diaphorina citri (Asian citrus psyllid) geographic populations, DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, 29(4), dsac027. https://doi.org/10.1093/dnares/dsac027 Chaverra-Rodriguez D., Bui M., Gilleland C.L., Rasgon J.L., and Akbari O.S., 2023, CRISPR-Cas9-mediated mutagenesis of the Asian citrus psyllid, Diaphorina citri, GEN Biotechnology, 2(4): 317-329. https://doi.org/10.1089/genbio.2023.0022 Chen K., Wang Y., Zhang R., Zhang H., and Gao C., 2019, CRISPR/Cas genome editing and precision plant breeding in agriculture, Annual Review of Plant Biology, 70: 667-697. https://doi.org/10.1146/annurev-arplant-050718-100049
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