International Journal of Horticulture, 2024, Vol.14, No.3, 142-155 http://hortherbpublisher.com/index.php/ijh 152 visible somatic mutations and demonstrating the feasibility of gene editing in this pest (Chaverra-Rodriguez et al., 2023). These innovations pave the way for more precise and efficient genetic modifications, potentially leading to the development of citrus varieties that are inherently resistant to ACP and the diseases it transmits, such as Huanglongbing (HLB). 7.2 Potential integration with other biotechnological approaches (e.g., RNAi, transgenics) Integrating CRISPR/Cas9 with other biotechnological approaches could enhance the effectiveness of pest management strategies. For instance, RNA interference (RNAi) has shown promise in silencing specific genes in ACP, leading to increased mortality or reduced transmission of HLB-causing bacteria (Yu and Killiny, 2018). Combining CRISPR/Cas9 with RNAi could allow for the simultaneous targeting of multiple genes, thereby increasing the robustness of pest resistance. Additionally, transgenic approaches that incorporate resistance traits into citrus germplasm could be complemented by CRISPR/Cas9 to fine-tune these traits, ensuring a more comprehensive defense against ACP and HLB (Yu and Killiny, 2018; Chen et al., 2021). 7.3 Long-term vision for sustainable citrus production and pest management The long-term vision for sustainable citrus production involves a multi-faceted approach that integrates advanced genetic tools with traditional pest management strategies. CRISPR/Cas9 technology offers a promising solution for developing citrus varieties that are resistant to ACP and HLB, potentially reducing the reliance on chemical insecticides, which are costly and often ineffective (Yu and Killiny, 2018; Tian et al., 2019; Jia et al., 2021). By fostering a deeper understanding of ACP biology and its interactions with citrus plants at the molecular level, researchers can develop more targeted and sustainable pest management strategies. Ultimately, the goal is to create a resilient citrus industry that can withstand the challenges posed by pests and diseases, ensuring the long-term viability of citrus production (Yu and Killiny, 2018; Chen et al., 2021; Chaverra-Rodriguez et al., 2023). 8 Concluding Remarks 8.1 Summary of key findings The research on using CRISPR/Cas9 gene editing technology to develop citrus germplasm resistant to the Asian citrus psyllid (ACP), Diaphorina citri, has shown promising advancements. The optimized methods for CRISPR/Cas9-based genetic modification in D. citri have successfully generated visible somatic mutations, indicating the feasibility of gene editing in this species (Huang et al., 2022; Chaverra-Rodrigue et al., 2023). Additionally, the BAPC-assisted CRISPR/Cas9 system has enabled heritable germline gene editing by delivering CRISPR components directly into adult ovaries, bypassing the need for embryonic injections (Hunter et al., 2018; Chaverra-Rodrigue et al., 2023). These breakthroughs are crucial for developing sustainable strategies to control ACP populations and, consequently, the spread of Huanglongbing (HLB) disease. 8.2 Implications for researchers, breeders, and policymakers For researchers, these findings open new avenues for studying the genetic basis of ACP resistance and developing targeted gene-editing strategies to mitigate the spread of HLB. The ability to perform heritable gene editing in ACP can lead to the creation of genetically modified psyllid populations that are less capable of transmitting the HLB pathogen (Hunter et al., 2018). Collaboration across disciplines, including genomics, plant pathology, entomology, and bioinformatics, will be essential to address the complex nature of ACP and HLB resistance (Tian et al., 2018; Hunter et al., 2020). For breeders, the integration of CRISPR/Cas9 technology into breeding programs can accelerate the development of HLB-resistant citrus varieties, ensuring the long-term sustainability of citrus production. Policymakers play a crucial role in shaping the regulatory landscape for gene-edited crops. Clear, science-based regulations that distinguish between traditional GMOs and CRISPR/Cas9-edited plants can facilitate the adoption of these technologies. Public engagement and education are also vital to build trust and acceptance among consumers. Transparent communication about the benefits, risks, and safety of CRISPR/Cas9 technology can help alleviate public concerns and promote informed decision-making.
RkJQdWJsaXNoZXIy MjQ4ODYzNA==